Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 1 , Pages 22-30, March 2006

Nanoprobes and nanobiosensors for monitoring and imaging individual living cells

Center for Advanced Biomedical Photonics, Advanced Biomedical Science and Technology Group, Life Sciences Division, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, Tennessee, USA

Received 8 September 2005; accepted 10 October 2005.

Abstract 

This article presents an overview of nanobiosensors and nanoprobes. The fabrication, operating principle, and applications of these systems and their capacity for in vivo analysis and optical imaging at the single-cell level are discussed. Recently the cross-disciplinary integration of nanotechnology, biology, and photonics has been revolutionizing important areas in molecular biology, especially diagnostics and therapy at the molecular and cellular levels. Nanobiosensors and nanoprobes are a relatively new class of biosensing and imaging devices that allow for analytical measurements in individual living cells. These devices have the capacity to sense individual chemical species in specific locations within a cell. This article discusses the usefulness and potential of these nanotechnology-based systems in biological research and their applications to monitoring individual living cells.

Key words: Nanobiosensor, Apoptosis, Nanoprobes, Surface-enhanced Raman scattering (SERS), Imaging

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 No financial conflict of interest was reported by the authors of this paper.

 The authors acknowledge the contribution of J.P. Alarie, G.D. Griffin, B.M. Cullum, and J.M. Song. This research is sponsored by the LDRD Project (Advanced Nanosensors), and by the Office of Biological and Environmental Research, U.S. Department of Energy under contract DE-AC05-00OR22725 managed by UT-Battelle, LLC. All authors have read and approved submission of the manuscript, and the manuscript has not been published and is not being considered for publication elsewhere in whole or part in any language except as an abstract.

PII: S1549-9634(06)00002-5

doi:10.1016/j.nano.2005.10.012

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 1 , Pages 22-30, March 2006