Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 1 , Pages 31-36 , March 2006

Recovery of elasticity of aged human epithelial cells in vitro

  • Igor Sokolov, PhD

      Affiliations

    • Department of Physics, Clarkson University, Potsdam, New York, USA
    • Department of Chemistry, Clarkson University, Potsdam, New York, USA
    • Corresponding Author InformationCorresponding author. Department of Physics, Clarkson University, Potsdam, NY 13699-5820 USA.
  • ,
  • Swaminathan Iyer, PhD

      Affiliations

    • Department of Physics, Clarkson University, Potsdam, New York, USA
  • ,
  • Craig D. Woodworth, PhD

      Affiliations

    • Department of Biology, Clarkson University, Potsdam, New York, USA

Received 11 July 2005 ,Accepted 23 December 2005.

References 

  1. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, et al. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proc Natl Acad Sci U S A. 1995;92:9363–9367
  2. Ulrich P, Zhang X. Pharmacological reversal of advanced glycation end-product-mediated protein crosslinking. Diabetologia. 1997;40:S157–S159
  3. Perry G, Smith MA. Active glycation in neurofibrillary pathology of Alzheimer's disease: N-(carboxymethyl) lysine and hexitol-lysine. Free Radical Biol Med. 2001;31:175–180
  4. Bucala R, Cerami A. Advanced glycosylation: chemistry, biology, and implications for diabetes and aging. Adv Pharmacol. 1992;23:1–34
  5. Vasan S, Zhang X, Kapurniotu A, Bernhagen J, Teichberg S, Basgen J, et al. An agent cleaving glucose-derived protein crosslinks in vitro and in vivo. Nature. 1996;382(6588):275–278
  6. Berdyyeva TK, Woodworth CD, Sokolov I. Human epithelial cells increase their rigidity with ageing in vitro: direct measurements. Phys Med Biol. 2005;50(1):81–92
  7. Berdyyeva T, Woodworth CD, Sokolov I. Visualization of cytoskeletal elements by the atomic force microscope. Ultramicroscopy. 2005;102(3):189–198
  8. Woodworth CD, Doniger J, Dipaolo JA. Immortalization of human foreskin keratinocytes by various human papillomavirus DNAs corresponds to their association with cervical carcinoma. J Virol. 1989;63:159–164
  9. Sneddon IN. The relation between load and penetration in the axisymmetric Boussinesq problem for a punch of arbitrary profile. Int J Eng Sci. 1965;3:47–57
  10. Tsukruk VV, Gorbunov VV, Huang Z, Chizhik SA. Dynamic microprobing of viscoelastic polymer properties. Polymer Int. 2000;5:441–447
  11. Vinckier A, Semenza G. Measuring elasticity of biological materials by atomic force microscopy. FEBS Lett. 1998;430(1-2):12–16
  12. Rotsch C, Braet F, Wisse E, Radmacher M. AFM imaging and elasticity measurements on living rat liver macrophages. Cell Biol Int. 1997;21:685–696
  13. Wu HW, Kuhn T, Moy VT. Mechanical properties of L929 cells measured by atomic force microscopy: effects of anticytoskeletal drugs and membrane crosslinking. Scanning. 1998;20:389–397
  14. Radmacher M. Review of AFM mechanical properties of the cells. Measuring the elastic properties of biological samples with the AFM. IEEE Eng Med Biol Mag. 1997;16:47–57
  15. Matzke R, Jacobson K, Radmacher M. Direct, high-resolution measurement of furrow stiffening during division of adherent cells. Nat Cell Biol. 2001;3:607–610
  16. Rao KMK, Currie MS, Padmanabhan J, Cohen HJ. Age-related changes in actin cytoskeleton and receptor expression in human leukocytes. J Gerontol. 1992;47:37–44
  17. Barrandon Y, Green H. Three clonal types of keratinocyte with different capacities for multiplication. Proc Natl Acad Sci USA. 1987;84(8):2302–2306

 No financial conflict of interest was reported by the authors of this paper.

PII: S1549-9634(06)00007-4

doi: 10.1016/j.nano.2005.12.002

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 1 , Pages 31-36 , March 2006