Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 2 , Pages 103-112 , June 2006

Fabrication of biofunctional nanomaterials via Escherichia coli OmpF protein air/water interface insertion/integration with copolymeric amphiphiles

  • Dean Ho, PhD

      Affiliations

    • Department of Mechanical and Aerospace Engineering, Henry Samueli School of Engineering and Applied Science, University of California at Los Angeles, Los Angeles, California
    • Corresponding Author InformationCorresponding author. Henry Samueli School of Engineering and Applied Science, University of California at Los Angeles, 7523 Boelter Hall, 420 Westwood Plaza, Los Angeles, California 90095.
  • ,
  • Stacy Chang, BS

      Affiliations

    • Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California at Los Angeles, Los Angeles, California
  • ,
  • Carlo D. Montemagno, PhD

      Affiliations

    • Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California at Los Angeles, Los Angeles, California

Received 23 September 2005 ,Accepted 22 December 2005.

References 

  1. Bogolmolni RA, Baker RA, Lozier RH. Light-driven proton translocations in Halobacterium halobium. Biochim Biophys Acta. 1976;440:68–78
  2. Stoeckenius W. Bacteriorhodopsin and the purple membrane in halobacteria. Biochim Biophys Acta. 1979;505:215–278
  3. Pitard B, Richard P, Dunach M, Rigaud JL. ATP synthesis by the F0F1 ATP synthase from thermophilic Bacillus PS3 reconstituted into liposomes with bacteriorhodopsin. 2. Relationships between proton motive force and ATP synthesis. Eur J Biochem. 1996;235:779–788
  4. Cowan SW, Garavito RM, Jansonius JN, Jenkins JA, Karlsson R, Konig N, et al. The structure of OmpF porin in a tetragonal crystal form. Structure. 1995;3:1041–1050
  5. Liu X, Ferenci T. An analysis of multifactorial influences on the transcriptional control of ompF and ompC porin expression under nutrient limitation. Microbiology. 2001;147:2981–2989
  6. Cowan SW, Schirmer T, Rummel G, Steiert M, Ghosh R, Pauptit RA, et al. Crystal structures explain functional properties of two E. coli porins. Nature. 1992;358:727–733
  7. Robertson K, Tieleman D. Orientation and interactions of dipolar molecules during transport through OmpF porin. FEBS Lett. 2002;528:53–57
  8. Phale S, Philippsen A, Widmer C, Phale V, Rosenbusch J, Schirmer T. Role of charged residues at the OmpF porin channel constriction probed by mutagenesis and simulation. Biochemistry. 2001;40:6319–6325
  9. Nardin C, Meier W. Hybrid materials from amphiphilic block copolymers and membrane proteins. Rev Mol Biotechnol. 2002;90:17–26
  10. Meier W, Nardin C, Winterhalter M. Reconstitution of membrane proteins in (polymerized) ABA-triblock copolymer membranes. Angew Chem Int Ed. 2000;39:4599–4602
  11. Ho D, Chu B, Schmidt JJ, Brooks EK, Montemagno CD. Hybrid protein/polymer biomimetic membranes. IEEE Proc Nano. 2003;2:379–382
  12. Ho D, Chu B, Schmidt JJ, Brooks EK, Montemagno CD. Hybrid protein-polymer biomimetic membranes. IEEE Trans Nano. 2004;3:256–263
  13. Ho D, Chen Y. Interfacing cellular systems with abiotic materials using composite collagen-block copolymer thin films. Bio-Nano-Information Fusion. 2005;1:1–4
  14. Rathman J, Sun P. Biocomposite films synthesized at a fluid/fluid interface. Faraday Discuss. 2005;129:193–203
  15. Ghannam MM, Mady MM, Khalil WA. Interaction of type-I collagen with phospholipid monolayer. Biophys Chem. 1999;80:31–40
  16. Heller A. Miniature biofuel cells. Phys Chem Chem Phys. 2004;6:209–216
  17. Ho D, Chu B, Lee H, Montemagno CD. Protein-driven energy transduction across polymeric biomembranes. Nanotechnology. 2004;15:1084–1094
  18. Pata V, Dan N. The effect of chain length on protein solubilization in polymer-based vesicles (polymersomes). Biophys J. 2003;85:2111–2118
  19. Discher BM, Won YY, Ege DS, Lee JCM, Bates FS, Discher DE, et al. Polymersomes: tough vesicles made from diblock copolymers. Science. 1999;284:1143–1146
  20. Aranda-Espinoza H, Bermudez H, Bates FS, Discher DE. Electromechanical limits of polymersomes. Phys Rev Lett 2001;87:208301–4.
  21. Discher BM, Bermudez H, Won YY, Hammer DA, Bates FS, Discher DE. Crosslinked copolymer vesicles with broadly tunable stability. J Phys Chem. 2002;B106:2848–2854
  22. Discher DE, Eisenberg A. Polymer vesicles. Science. 2002;297:967–973
  23. Graff A, Sauer M, van Gelder P, Meier W. Virus-assisted loading of polymeric nanocontainers. Proc Natl Acad Sci. 2002;99:5064–5068

 The authors gratefully thank the T. Schirmer group at the Division of Structural Biology, Biozentrum, University of Basel, Basel, Switzerland for the OmpF plasmid.

PII: S1549-9634(06)00009-8

doi: 10.1016/j.nano.2005.12.004

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 2 , Pages 103-112 , June 2006