Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 3 , Pages 177-181, September 2006

Advantages of ultrashort phase-shaped pulses for selective two-photon activation and biomedical imaging

  • Laura T. Schelhas

      Affiliations

    • Department of Chemistry, Michigan State University, East Lansing, Michigan, USA
    • ,
  • Janelle C. Shane

      Affiliations

    • Department of Electrical and Computer Engineering, Michigan State University, East Lansing, Michigan, USA
    • ,
  • Marcos Dantus, PhD

      Affiliations

    • Department of Physics and Astronomy, Michigan State University, East Lansing, Michigan, USA
    • Corresponding Author InformationCorresponding author. Department of Chemistry, Michigan State University, East Lansing, Michigan, USA.

Received 22 May 2006; accepted 19 July 2006.

Abstract 

Two-photon excitation using ultrashort laser pulses can selectively activate nanoparticles or excite fluorophores within thick biological samples. We show how the use of methods such as multiphoton intrapulse interference phase scan (MIIPS) to compensate phase distortions caused by microscope objectives with a high numerical aperture increases signal intensity and reproducibility in two-photon imaging. Using phase shaping of our compensated pulses, we demonstrate selective excitation of fluorophores within a mouse kidney sample, increasing the contrast between different subcellular structures compared to unshaped pulses.

Key words: Femtosecond laser, Pulse shaping, Two-photon imaging, Selective excitation

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PII: S1549-9634(06)00105-5

doi:10.1016/j.nano.2006.07.007

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 2, Issue 3 , Pages 177-181, September 2006

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