Development of hyaluronic acid–Fe2O3 hybrid magnetic nanoparticles for targeted delivery of peptides

Kumar, Arun; Sahoo, Bishwabhusan; Montpetit, Alison; Behera, Sumita; Lockey, Richard F.; Mohapatra, Shyam S.

doi:10.1016/j.nano.2007.03.001

« Previous Next »Nanomedicine: Nanotechnology, Biology and Medicine
Volume 3, Issue 2 , Pages 132-137, June 2007

Development of hyaluronic acid–Fe₂O₃ hybrid magnetic nanoparticles for targeted delivery of peptides

Arun Kumar, PhD
- Division of Allergy and Immunology, Department of Internal Medicine, College of Medicine, University of South Florida, Tampa, Florida
Bishwabhusan Sahoo, PhD
- Division of Allergy and Immunology, Department of Internal Medicine, College of Medicine, University of South Florida, Tampa, Florida
- Transgenex Nanobiotech, Inc., Tampa, Florida
Alison Montpetit, RN, MS
- Division of Allergy and Immunology, Department of Internal Medicine, College of Medicine, University of South Florida, Tampa, Florida
- Transgenex Nanobiotech, Inc., Tampa, Florida
Sumita Behera, PhD
- Transgenex Nanobiotech, Inc., Tampa, Florida
Richard F. Lockey, MD
- Division of Allergy and Immunology, Department of Internal Medicine, College of Medicine, University of South Florida, Tampa, Florida
- VA Hospital Research, James A. Haley Hospital, Tampa, Florida
Shyam S. Mohapatra, PhD
- Division of Allergy and Immunology, Department of Internal Medicine, College of Medicine, University of South Florida, Tampa, Florida
- Transgenex Nanobiotech, Inc., Tampa, Florida
- Corresponding author. Dr. Shyam S. Mohapatra, Department of Internal Medicine, University of South Florida, 12901 Bruce B Downs Blvd., MDC-19, Tampa, FL 33612, USA.

Received 15 June 2006; accepted 2 March 2007.

Abstract

Novel hybrid nanoparticles comprised of hyaluronic acid (HA) and iron oxide were synthesized and characterized for the first time with the average diameter of less than 160 nm. The iron oxide (Fe₂O₃) particles are hybridized between HA layers by electrostatic interactions between the positive surface charge of the Fe₂O₃ nanoparticles and the negative charge of the carboxylate groups of HA, forming a corral-like structure. The particles were also characterized by FTIR and NMR to verify the hybridization. The particles were tested for their ability to deliver peptides to the cells using HEK293 and A549 cells. Results show that these particles delivered peptides at about 100% level. These HA-iron oxide nanoparticles are expected to be useful in developing effective tissue and cell targeting systems.

Key words: Hyaluronic acid, Iron oxide particles, Hybrid nanoparticles, Drug targeting systems and cell targeting