Original ArticleClinical NanomedicineSynthesis and effect of silver nanoparticles on the antibacterial activity of different antibiotics against Staphylococcus aureus and Escherichia coli
Section snippets
Synthesis of Ag-NPs
Colloidal Ag-NPs solution was prepared following the method already described [8], [9]. Müller-Hinton medium was prepared, sterilized, and inoculated with a fresh growth of test strain. The cultured flasks were incubated at 37°C for 24 hours. After the incubation time the culture was centrifuged at 12,000 rpm and the supernatant was used for the synthesis of Ag-NPs. K. pneumoniae culture supernatant was separately added to the reaction vessels containing silver nitrate at a concentration of 10–3
Results and discussion
Ag-NPs were synthesized from Ag+ ions by treating the supernatant of K. pneumonia. The appearance of a yellowish brown color in the reaction vessels suggested the formation of Ag-NPs [10]. The supernatant of the K. pneumonia culture changed the solution to a brownish color upon the completion of the 5-minute reaction with Ag+ (Figure 1). The Ag-NPs were characterized by UV-vis spectroscopy. The technique outlined above has proved to be very useful for the analysis of nanoparticles [11], [12],
Acknowledgments
This research was supported by the Medical Nanotechnology Research Center, Faculty of Pharmacy, Medical Sciences/University of Tehran, Tehran, Iran.
References (16)
- et al.
The study of antimicrobial activity and preservative effects of nanosilver ingredient
Electrochimica Acta
(2005) Resisting resistance: new chemical strategies for battling superbugs
Chem Biol
(2000)Bacterial resistance to antibiotics: enzymatic degradation and modification
Adv Drug Deliv Rev
(2005)- et al.
Extracellular biosynthesis of silver nanoparticles using the fungus Fusarium oxysporum
Colloid Surf B
(2003) - et al.
pH-dependent changes in the optical properties of carboxylic acid derivatized silver colloidal particles
Colloid Surf A
(1997) - et al.
Electrical transport studies of Ag nanocrystallites embedded in glass matrix
Physics B
(2001) - et al.
Antimicrobial effects of metal ions (Ag+, Cu2+, Zn2+) in hydroxyapatite
J Mater Sci Mater Med
(1998) - et al.
Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3
Nanotechnology
(2003)
Cited by (0)
No conflict of interest was reported by the authors of this paper.