Comparison between titanium and anatase miRNAs regulation

Palmieri, Annalisa; Brunelli, Giorgio; Guerzoni, Laura; Muzio, Lorenzo Lo; Scarano, Antonio; Rubini, Corrado; Scapoli, Luca; Martinelli, Marcella; Pezzetti, Furio; Carinci, Francesco

doi:10.1016/j.nano.2007.03.004

« Previous Next »Nanomedicine: Nanotechnology, Biology and Medicine
Volume 3, Issue 2 , Pages 138-143, June 2007

Comparison between titanium and anatase miRNAs regulation

Annalisa Palmieri, PhD
- Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy
Giorgio Brunelli, MD
- Department of Discipline Medicine Chirurgiche Comunicazione Comportamento, Section of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy
Laura Guerzoni, PhD
- Department of Discipline Medicine Chirurgiche Comunicazione Comportamento, Section of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy
Lorenzo Lo Muzio, MD
- Dental Clinic, University of Foggia, Foggia, Italy
Antonio Scarano, DDS
- Dental Clinic, University of Chieti, Chieti, Italy
Corrado Rubini, MD
- Department of Neurosciences, Institute of Pathologic Anatomy and Histopathology, Polytecnic University of the Marche, Ancona, Italy
Luca Scapoli, PhD
- Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy
Marcella Martinelli, PhD
- Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy
Furio Pezzetti, PhD
- Institute of Histology, University of Bologna and Center of Molecular Genetics, CARISBO Foundation, Bologna, Italy
Francesco Carinci, MD
- Department of Discipline Medicine Chirurgiche Comunicazione Comportamento, Section of Maxillofacial Surgery, University of Ferrara, Ferrara, Italy
- Corresponding author. Prof. Francesco Carinci, University of Ferrara, DMCCC, Corso Giovecca 203, 44100, Ferrara, Italy.

Received 9 January 2007; accepted 2 March 2007.

Abstract

Titanium is the gold standard among materials used for prosthetic devices because of its good mechanical and chemical properties. There are three allotropic forms of titanium dioxide: brookite, rutile, and anatase. Anatase can be prepared as a colloidal suspension and used to coat surfaces. Anatase coating (AC) can potentially have biological effects and specifically can induce bone formation. To obtain more information about the osteogenic effect of AC in comparison to to titanium we used microRNA (miRNA) microarray techniques to investigate the translation regulation in osteoblasts exposed to both titanium and AC. There were three upregulated miRNAs (mir-1, mir-34c, mir-210) and eight downregulated miRNAs (mir-23b, mir-377, mir-22, mir-93, mir-422b, mir-17-5p, mir-24, mir-130b) for false discovery rate = 0 and score >3. The data reported are relevant to understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

Key words: Titanium, miRNA, Translation, Osseointegration, Implant