Original Article: Drug Delivery NanomedicineMannosylated gelatin nanoparticles bearing an anti-HIV drug didanosine for site-specific delivery
Section snippets
Materials
DDI was obtained as a gift sample from the Hetro Laboratories (Hyderabad, India). 3-(4,5-dimetylthiazol-2-2-y1)-2,5-diphenyl) tetrazolium bromide (MTT), 6-carboxyfluorescein (6-CF), Triton X-100, Sephadex G-50, cysteine, and mannose were procured from Sigma Chemicals (St Louis, MO). Gelatin, Hank’s balanced salt solution, HEPES buffer medium, and dialysis membrane (MWCO 12–14 kDa) were procured from Himedia (Mumbai, India). Glutaraldehyde, acetone, methanol, and acetonitrile were purchased from
Preparation and characterization of mannosylated gelatin nanoparticles
G-NPs were prepared using modified two-step desolvation technique using acetone and glutaraldehyde as desolvating agent and cross-linking agent, respectively. The G-NPs prepared by the two-step desolvation method showed a high stability in both water and cell medium. The preparation of G-NPs using a two-step desolvation procedure showed much less aggregation as compared with the earlier described one-step desolvation method.28 The sediment collected following the first desolvation step showed
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2021, Journal of Pharmaceutical SciencesCitation Excerpt :This could be attributed to the fact that mannan blocks the mannose receptors, and thus reduces uptake of Mn–C6-SLNs. Hence, the current data using fluorescence microscopy endorse that the uptake of SLNs is via mannose receptor-mediated endocytosis, and in agreement to the results of quantitative analysis using FACS.74 [ Fig. 5 near here].
The authors are thankful to Hetro Laboratories (Hyderabad, India) for a gift sample of didanisone. The authors are grateful to the All India Institute of Medical Sciences, New Delhi, for providing the SEM facility.