Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 1 , Pages 49-56 , March 2008

Fluorescence intensity and lifetime imaging of free and micellar-encapsulated doxorubicin in living cells

  • Xiaowen Dai, BEng

      Affiliations

    • Department of Chemical Engineering, University of Cambridge, Cambridge
  • ,
  • Zhilian Yue, PhD

      Affiliations

    • Department of Chemical Engineering, University of Cambridge, Cambridge
  • ,
  • Mark E. Eccleston, PhD

      Affiliations

    • Vivamer Ltd., Cambridge, United Kingdom
  • ,
  • Johannes Swartling, PhD

      Affiliations

    • Department of Chemical Engineering, University of Cambridge, Cambridge
  • ,
  • Nigel K.H. Slater, PhD

      Affiliations

    • Department of Chemical Engineering, University of Cambridge, Cambridge
  • ,
  • Clemens F. Kaminski, PhD

      Affiliations

    • Department of Chemical Engineering, University of Cambridge, Cambridge
    • Corresponding Author InformationCorresponding author.

References 

  1. Vasey PA, Kaye SB, Morrison R, Twelves C, Wilson P, Duncan R, et al. Phase I clinical and pharmacokinetic study of PK1 [N-(2-hydroxypropyl)methacrylamide copolymer doxorubicin]: first member of a new class of chemotherapeutic agents—drug-polymer conjugates. Clin Cancer Res. 1999;5:83–94
  2. Lukyanov AN, Gao Z, Mazzola L, Torchilin VP. Polyethylene glycol-diacyllipid micelles demonstrate increased accumulation in subcutaneous tumors in mice. Pharm Res. 2002;19:1424–1429
  3. Kakizawa Y, Kataoka K. Block copolymer micelles for delivery of gene and related compounds. Adv Drug Deliv Rev. 2002;54:20–222
  4. Tomlinson R, Heller J, Brocchini S, Duncan R. Polyacetal-doxorubicin conjugates designed for pH-dependent degradation. Bioconjug Chem. 2003;14:1096–1106
  5. Lukyanov AN, Gao Z, Torchilin VP. Micelles from polyethylene glycol/phosphatidylethanolamine conjugates for tumor drug delivery. J Control Release. 2003;91:97–102
  6. Kaneda Y, Tsutsumi Y, Yoshioka Y, Kamada H, Yamamoto Y, Kodaira H. The use of PVP as a polymeric carric to improve the plasma half-life of drugs. Biomaterials. 2004;25:3259–3266
  7. Ahmed F, Discher DE. Self-porating polymersomes of PEG-PLA and PEG-PCL: hydrolysis-triggered controlled release vesicles. J Control Release. 2004;96:37–53
  8. Veronese FM, Schiavon O, Pasut G, Mendichi R, Andersson L, Tsirk A, et al. PEG-doxorubicin conjugates: influence of polymer structure on drug release, in vitro cytotoxicity, biodistribution, and antitumor activity. Bioconjug Chem. 2005;16:775–784
  9. Bader H, Ringsdorf H, Schmidt B, Water soluble polymers in medicine. Angew Chem. 1984;123-124:457-85.
  10. Kabanov AV, Chekhonin VP, Alakhov VY, Batrakova EV, Lebedev AS, Melik-Nubarov NS, et al. The neuroleptic activity of haloperidol increases after its solubilization in surfactant micelles: micelles as microcontainers for drug targeting. FEBS Lett. 1989;258:343–345
  11. Yokoyama M, Satoh A, Sakurai Y, Okano T, Matsumura Y, Kakizoe T, et al. Incorporation of water-insoluble anti-cancer drug into polymeric micelles and control of their particle size. J Control Release. 1998;55:219–229
  12. Calleja V, Ameer-Beg SM, Vojnovic B, Woscholski R, Downward J, Larijani B. Monitoring conformational changes of proteins in cells by fluorescence lifetime imaging microscopy. J. Biochem. 2003;372:33–40
  13. Vermeer JEM, Van Munster EB, Vischer NO, Gadella TWJ. Probing plasma membrane microdomains in cowpea protoplasts using lipidated GFP-fusion proteins and multimode FRET microscopy. J Microsc. 2003;214:190–200
  14. Lin HJ, Herman P, Lakowicz JR. Fluorescence lifetime-resolved pH imaging of living cells. Cytometry. 2003;52A:77–89
  15. Clayton AHA, Hanley QS, Arndt-Jovin DJ, Subramaniam V, Jovin TM. Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM). J. Biophys. 2002;83:1631–1649
  16. Campbell A, Uttamchandani D. Optical dissolved oxygen lifetime sensor based on sol-gel immobilization. Science, Measurement and Technology, IEE Proc. 2004;151:291–297
  17. Hanley QS, Subramaniam V, Arndt-Jovin DJ, Jovin TM. Fluorescence lifetime imaging: multi-point calibration, minimum resolvable differences, and artifact suppression. Cytometry. 2001;43:248–260
  18. Eccleston ME, Kuiper M, Gilchrist FM, Slater NKH. pH-responsive pseudo-peptides for cell membrane disruption. J Control Release. 2000;69:297–307
  19. Swartling J, Bassi A, D'Andrea C, Pifferi A, Torricelli A, Cubeddu R. Dynamic time-resolved diffuse spectroscopy based on supercontinuum light pulses. Appl Opt. 2005;44:4684–4692
  20. Elder AD, Matthews SM, Swartling J, Yunus K, Frank JH, Brennan CM, et al. Application of fluorescence lifetime imaging microscopy as a quantitative analytical tool for microfluidic devices. Opt Express. 2006;14:5456–5467
  21. Elder AD, Frank JH, Swartling J, Dai X, Kaminski CF. Calibration of a wide-field frequency-domain fluorescence lifetime microscopy system using light emitting diodes as light sources. J Microsc. 2006;224:166–180
  22. Claires JB, Dattagupta N, Crothers DM. Studies on interaction of anthracycline antibiotics and deoxyribonucleic acid: equilibrium binding studies on the interaction of daunomycin with deoxyribonucleic acid. Biochemistry. 1982;21:3933–3940
  23. Ross WE. DNA topoisomerases as targets for cancer therapy. Biochem Pharmacol. 1985;34:4191–4195
  24. Bodley A, Liu LF, Israel M, Seshadri R, Koseki Y, Giuliani FC, et al. DNA topoisomerase II-mediated interaction of doxorubicin and daunorubicin congeners with DNA. Cancer Res. 1989;49:5969–5978
  25. Ashikawa I, Kinosita K, Ikegami A. Increased stability of the higher order structure of chicken erythrocyte chromatin: nanosecond anisotropy studies of intercalated ethidium. Biochemistry. 1985;24:1291–1297
  26. Malatesta V, Andreoni A. Dynamics of anthracyclines/DNA interaction: a laser time-resolved fluorescence study. Photochem Photobiol. 1988;48:409–415

 No conflict of interest was reported by the authors of this paper.

PII: S1549-9634(07)00254-7

doi: 10.1016/j.nano.2007.12.002

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 1 , Pages 49-56 , March 2008