N-hexanoyl chitosan-stabilized magnetic nanoparticles: enhancement of adenoviral-mediated gene expression both in vitro and in vivo

Bhattarai, Shanta Raj; Kim, Sun Young; Jang, Kyu Yun; Lee, Ki Chang; Yi, Ho Keun; Lee, Dae Yeol; Kim, Hak Yong; Hwang, Pyoung Han

doi:10.1016/j.nano.2008.02.001

« Previous Next »Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 2 , Pages 146-154, June 2008

N-hexanoyl chitosan-stabilized magnetic nanoparticles: enhancement of adenoviral-mediated gene expression both in vitro and in vivo

Shanta Raj Bhattarai, PhD
- Department of Pharmaceutical Sciences, Wayne State University, Detroit, Michigan, USA
Sun Young Kim, PhD
- Department of Pediatrics and Research Institute of Clinical Medicine, School of Medicine, Chonbuk National University, Jeonju, South Korea
Kyu Yun Jang, MD, PhD
- Department of Pathology, School of Medicine, Chonbuk National University, Jeonju, South Korea
Ki Chang Lee, PhD
- Department of Veterinary Radiology, School of Veterinary Medicine, Chonbuk National University, Jeonju, South Korea
Ho Keun Yi, PhD
- Department of Biochemistry, School of Dentistry, Chonbuk National University, Jeonju, South Korea
Dae Yeol Lee, MD, PhD
- Department of Pediatrics and Research Institute of Clinical Medicine, School of Medicine, Chonbuk National University, Jeonju, South Korea
Hak Yong Kim, PhD
- Department of Textile Engineering, Chonbuk National University, Jeonju, South Korea
Pyoung Han Hwang, MD, PhD
- Department of Pediatrics and Research Institute of Clinical Medicine, School of Medicine, Chonbuk National University, Jeonju, South Korea
- Corresponding author. Department of Pediatrics, Chonbuk National University, 634-18 Geumam-dong, Deokjin-gu, Jeonju, Jeonbuk 561-712, Korea.

Received 25 August 2007; accepted 8 February 2008. published online 31 March 2008.

Abstract

We developed hexanoyl chloride–modified chitosan (Nac-6) stabilized iron oxide nanoparticles (Nac-6-IOPs) as magnetic nanoparticles for viral gene (Ad/LacZ) delivery via magnetofection. This vector, Nac-6-IOPs/Ad/LacZ, binds to K562 cells in the presence of external magnetic fields and results in enhanced expression of the transgene in those cells that do not exhibit the coxsackie-adenovirus receptor (CAR). Our results demonstrate that Nac-6-IOPs/Ad/LacZ is able to transduce K562 cells specifically with reduced infection of CAR^– cells. The dramatic enhancement in intracellular trafficking of the adenovirus without genetically modified vesicles can lead to enhanced nuclear transfer, especially in CAR^– cells. In vivo magnetofection results also clearly demonstrated that the present Nac-6-IOPs could be applied in other cell lines. Whether cells or organs, in the presence of magnetic fields Nac-6-IOPs/Ad/LacZ has high transduction efficiency. The newly formulated Nac-6-IOPs, introduced by magnetofection, provide a high-throughput gene screening both in vitro and in vivo.

Key words: Magnetofection, Gene, Vectors, Magnetic nanoparticles, Chitosan, Iron oxide, Virus