Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 2 , Pages 98-105 , June 2008

Methods for purifying and detoxifying sodium dodecyl sulfate–stabilized polyacrylate nanoparticles

  • Julio C. Garay-Jimenez, BS

      Affiliations

    • Department of Chemistry, Center for Molecular Diversity in Drug Design, Discovery, and Delivery, University of South Florida, Tampa, Florida, USA
    • ,
  • Ashley Young, BS

      Affiliations

    • Nanopharma Technologies, Inc., Tampa, Florida, USA
    • ,
  • Danielle Gergeres

      Affiliations

    • Department of Chemistry, Center for Molecular Diversity in Drug Design, Discovery, and Delivery, University of South Florida, Tampa, Florida, USA
    • ,
  • Kerriann Greenhalgh, BS, PhD

      Affiliations

    • Department of Chemistry, Center for Molecular Diversity in Drug Design, Discovery, and Delivery, University of South Florida, Tampa, Florida, USA
    • ,
  • Edward Turos, BS, PhD

      Affiliations

    • Department of Chemistry, Center for Molecular Diversity in Drug Design, Discovery, and Delivery, University of South Florida, Tampa, Florida, USA
    • Corresponding Author InformationCorresponding author. Center for Molecular Diversity in Drug Design, Discovery, and Delivery, Department of Chemistry CHE 205, 4202 East Fowler Avenue, University of South Florida, Tampa, FL 33620, USA.

Received 19 October 2007 ,Accepted 20 March 2008.

References 

  1. Spellberg B, Powers J, Brass E, Miller LG, Edwards JE. Trends in antimicrobial drug development: implication for the future. Clin Infect Dis. 2004;178:1279–1286
  2. Leeb M. Antibiotics: a shot in the arm. Nature. 2004;431:892–893
  3. Weber JT, Courvalin P. An emptying quiver: antimicrobial drugs and resistance. Emerg Infect Dis. 2005;11:791–793
  4. Harbarth S, Samore M. Antimicrobial resistance determinants and future control. Emerg Infect Dis. 2001;11:794–801
  5. DiMassa JA, Hansen RW, Grabowski HG. The price of innovation: new estimates of drug development cost. J Health Econ. 2003;22:151–185
  6. Gilbert DN, Edwards JE. Is there hope for the prevention of future antimicrobial shortages?. Clin Infect Dis. 2002;35:215–216
  7. Turos E, Shim JY, Wang Y, Greenhalgh K, Reddy GS, Dickey S, et al. Antibiotic-conjugated polyacrylate nanoparticles: new opportunities for development of anti-MRSA agents. Bioorg Med Chem Lett. 2007;17:53–56
  8. Abeylath S, Turos E. Glycosylated polyacrylate nanoparticles by emulsion polymerization. Carbohydr Polym. 2007;70:32–37
  9. Turos E, Reddy GSK, Greenhalgh K, Ramaraju P, Abeylath SC, Jang S, et al. Penicillin-bound polyacrylate nanoparticles: restoring the activity of β-lactam antibiotics against MRSA. Bioorg Med Chem Lett. 2007;17:3468–3472
  10. Turos E, Shim JY. Nanoparticles for drug delivery. PCT Int. Appl. 2005, WO 2005020933.
  11. Alleman E, Doelker E, Gurny R. Drug loaded poly(lactic acid) nanoparticles produced by a reversible salting-out process: purification on an injectable dosage. Eur J Pharm Biopharm. 1993;39:13–18
  12. Beck P, Scherer D, Kreuter J. Separation of drug-loaded nanoparticles from free drug by gel filtration. J Microencapsul. 1990;7:491–496
  13. Verhulst C, Coiffard C, Coiffard L, Rivalland P, De Roeck-Holzhauer Y. In vitro correlation between two colorimetric assays and the pyruvic acid consumption by fibroblasts cultured to determine the sodium laurysulfate cytotoxicity. J Pharmacol Toxicol Methods. 1998;39:143–146
  14. Mueller R, Ruehl D, Runge S, Schulze-Forster K, Mehnert W. Cytotoxicity of solid lipid nanoparticles as a function of the lipid matrix and the surfactant. Pharma Res. 1997;14:458–462
  15. Babich H, Babich J. Sodium lauryl sulfate and triclosan: in vitro cytotoxicity studies in gingival cells. Toxicol Lett. 1997;91:189–196
  16. Craig S, Newby C, Barr R, Greaves M, Mallet A. Cytokine release and cytotoxicity in human keratinocytes and fibroblast induced by phenols and sodium dodecyl sulfate. J Invest Dermatol. 2000;115:292–298
  17. Ruissen F, Carroll J, Vander Valk P, Schalkwijk J. Differential effects of detergents on keratinocyte gene expression. J Invest Dermatol. 1998;110:358–363
  18. Törmä H, Geijer S, Gester T, Alpholm K, Berne B, Lindberg M. Variations in the mRNA expression of inflammatory mediators, markers of differentiation and lipid-metabolizing enzymes caused by sodium lauryl sulphate in cultured human keratinocytes. Toxicol in Vitro. 2006;20:472–479
  19. Krebs F, Miller S, Catalone B, Welsh P, Malamud D, Howett M, et al. Sodium dodecyl sulfate and C31G as microbicidal alternatives to nonoxynol 9: comparative sensitivity of primary human vaginal keratinocytes. Antimicrob Agents Chemother. 2000;44:1954–1960
  20. Varani J, Astrom A, Griffiths C, Voorhees J. Induction of proliferation of growth-inhibited keratinocytes and fibroblasts in monolayer culture by sodium lauryl sulfate: comparison with all-trans retinoic acid. J Invest Dermatol. 1991;97:917–921
  21. Wei T, Geijer S, Lindberg M, Berne B, Törmä H. Detergents with different chemical properties induce variable degree of cytotoxicity and mRNA expression of lipid-metabolizing enzymes and differentiation markers in cultured keratinocytes. Toxicol in Vitro. 2006;20:1387–1394
  22. National Committee for Clinical Laboratory Standards . NCCLS methods for dilution of antimicrobial susceptibility test for bacteria that grow aerobically. NCCLS Document M7-A4. 1997;Vol. 17
  23. Murakami H, Kobayashi H, Takeuchi H, Kawashima Y. Further application of a modified spontaneous emulsification solvent diffusion method to various types of PLGA and PLA polymers for preparation of nanoparticles. Powder Technol. 2000;107:137–143
  24. Kreuse HJ, Schwartz A, Rohdewald P. Interfacial polymerization, a useful method for the preparation of poly(methyl cyanoacrylate) nanoparticles. Drug Dev Ind Pharm. 1986;12:527–552
  25. Zahka L, Mir L. Ultrafiltration of latex emulsions. Chem Eng Prog. 1977;73:53–55
  26. Kwon HY, Lee JY, Choi SW, Jang J, Kim JH. Preparation of PLGA nanoparticles containing estrogen by emulsification-diffusion method. Colloids Surf A Physicochem Eng Asp. 2001;182:123–130
  27. Dalwadi G, Heather A, Benson E, Chen Y. Comparison of diafiltration and tangential flow filtration for purification of nanoparticle suspensions. Pharm Res. 2005;22:2152–2162
  28. Liwarska-Bizukojc E, Miksch K, Malachowska-Jutsz A, Kalka J. Acute toxicity and genotoxicity of five selected anionic and nonionic surfactants. J Chemosphere. 2005;58:1249–1253

 The National Institutes of Health (R01 AI01535) and the National Science Foundation (NSF 0419903, NSF 0620572), US Department of Homeland Security (fellowship to K.G.), University of South Florida and the Florida Center of Excellence in Biomolecular Identification and Targeted Therapeutics (for a Graduate Multidisciplinary Scholarship to J.G.), and the University of South Florida Office of Technology Development (for a Florida High Tech Corridor matching grant) all provided support to this study.

 Conflict of interest: Edward Turos is co-inventor on a US patent application by the University of South Florida for the polyacrylate nanoparticle antibiotics, the subject of this publication. Dr. Turos is also co-founder, chief scientific advisor, and shareholder of Nanopharma Technologies, Inc., a University of South Florida spin-out company. Nanopharma Technologies, Inc., has licensed the nanoparticles technology from University of South Florida for potential commercial development.

PII: S1549-9634(08)00037-3

doi: 10.1016/j.nano.2008.03.004

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 2 , Pages 98-105 , June 2008

Article Tools

* Image Usage & Resolution