Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 3 , Pages 208-214, September 2008

Uptake of oleoyl-chitosan nanoparticles by A549 cells

  • Jing Zhang, PhD

      Affiliations

    • College of Marine Life Science, Ocean University of China, Qingdao, P.R. China
    • ,
  • Xi Guang Chen, PhD

      Affiliations

    • College of Marine Life Science, Ocean University of China, Qingdao, P.R. China
    • Corresponding Author InformationCorresponding author. College of Marine Life Science, Ocean University of China, Qingdao, P.R. China.
    • ,
  • Wei Bing Peng, PhD

      Affiliations

    • College of Medicine and Pharmacy, Ocean University of China, Qingdao, P.R. China
    • ,
  • Cheng Sheng Liu, PhD

      Affiliations

    • College of Marine Life Science, Ocean University of China, Qingdao, P.R. China

Received 24 December 2007; accepted 20 March 2008. published online 28 May 2008.

Abstract 

The aim of the present study was to evaluate cellular uptake of oleoyl-chitosan (OCH) nanoparticles by using A549 cells, a human lung carcinoma cell line, for drug and gene delivery applications. In this study, self-assembled OCH nanoparticles encapsulating a fluorescent marker molecule, fluorescein isothiocyanate (FITC), were prepared and characterized. The effects of particle size, concentration, and incubation time on the cellular uptake of the nanoparticles (FITC-OCH nanoparticles) were quantified by spectrofluorometric measurement and confirmed using fluorescence microscopy studies. The nanoparticles were taken up by the cells, and levels of binding and uptake increased with the decrease of particle size and the increase of particle concentration and incubation time. These results implied that the OCH nanoparticles have great potential to be applied as a drug carrier system to deliver drugs into the cells.

Key words: A549 cells, Cellular uptake, Fluorescein isothiocyanate (FITC), Nanoparticles

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 This work was supported by grants from National Natural Science Foundation of China (NSFC) (30770582) and International Science and Technology Cooperation Project (ISTCP) (2006DFA33150).

PII: S1549-9634(08)00040-3

doi:10.1016/j.nano.2008.03.006

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 3 , Pages 208-214, September 2008

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