Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 4 , Pages 340-349 , December 2008

Ocular nanoparticle toxicity and transfection of the retina and retinal pigment epithelium

  • Tarl W. Prow, PhD

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • Imran Bhutto, MD, PhD

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • Sahng Y. Kim, MD

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • Rhonda Grebe, BS

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • Carol Merges, MS

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • D. Scott McLeod

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • Koichi Uno, MD, PhD

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • Mohamed Mennon, MD

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • ,
  • Li Rodriguez, PhD

      Affiliations

    • Food and Drug Administration, Rockville, Maryland
    • ,
  • Kam Leong, PhD

      Affiliations

    • Duke University, Durham, North Carolina, USA
    • ,
  • Gerard A. Lutty, PhD

      Affiliations

    • The Wilmer Ophthalmological Institute, The Johns Hopkins University, Baltimore, Maryland
    • Corresponding Author InformationCorresponding author. Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21287-9115, USA.

Received 3 August 2007 ,Revised 19 February 2008 ,Accepted 6 June 2008.

References 

  1. Lynch SS, Cheng CM. Bevacizumab for neovascular ocular diseases. Ann. Pharmacotherapy. 2007;41:614–625
  2. Ng EW, Adamis AP. Anti-VEGF aptamer (pegaptanib) therapy for ocular vascular diseases. Ann N Y Acad Sci. 2006;1082:151–171
  3. Enriquez de Salamanca A, Diebold Y, Calonge M, Garcia-Vazquez C, Callejo S, Vila A, et al. Chitosan nanoparticles as a potential drug delivery system for the ocular surface: toxicity, uptake mechanism and in vivo tolerance. Invest Ophthalmol Vis Sci. 2006;47:1416–1425
  4. Jiang X, Dai H, Leong KW, Goh SH, Mao HQ, Yang YY. Chitosan-g-PEG/DNA complexes deliver gene to the rat liver via intrabiliary and intraportal infusions. J Gene Med. 2006;8:477–487
  5. Lee D, Zhang W, Shirley SA, Kong X, Hellermann GR, Lockey RF, et al. Thiolated chitosan/DNA nanocomplexes exhibit enhanced and sustained gene delivery. Pharm Res. 2007;24:157–167
  6. Leong KW, Mao HQ, Truong-Le VL, Roy K, Walsh SM, August JT. DNA-polycation nanospheres as non-viral gene delivery vehicles. J Control Release. 1998;53:183–193
  7. Wong K, Sun G, Zhang X, Dai H, Liu Y, He C, et al. PEI-g-chitosan, a sb:volume-nrnovel gene delivery system with transfection efficiency comparable to polyethylenimine in vitro and after liver administration in vivo. Bioconjug Chem. 2006;17:152–158
  8. Zhang Y, Chen J, Zhang Y, Pan Y, Zhao J, Ren L, et al. A novel PEGylation of chitosan nanoparticle for gene delivery. Biotechnol Appl Biochem. 2007;46:197–204
  9. Sander SV, Bondarchuk OI, Pentiuk AA. Effect of polysorb on adhesion of a gauze dressing to the wound surface. Klin Khir. 1992;(1):22–23(Russian)
  10. Dang JM, Sun DD, Shin-Ya Y, Sieber AN, Kostuik JP, Leong KW. Temperature-responsive hydroxybutyl chitosan for the culture of mesenchymal stem cells and intervertebral disk cells. Biomaterials. 2006;27:406–418
  11. Wen J, Mao HQ, Li W, Lin KY, Leong KW. Biodegradable polyphosphoester micelles for gene delivery. J Pharm Sci. 2004;93:2142–2157
  12. Thorek DL, Chen AK, Czupryna J, Tsourkas A. Superparamagnetic iron oxide nanoparticle probes for molecular imaging. Ann Biomed Eng. 2006;34:23–38
  13. Prow T, Smith JN, Grebe R, Salazar JH, Wang N, Kotov N, et al. Construction, gene delivery, and expression of DNA tethered nanoparticles. Mol Vis. 2006;12:606–615
  14. Prow T, Grebe R, Merges C, Smith JN, McLeod DS, Leary JF, et al. Nanoparticle tethered antioxidant response element as a biosensor for oxygen induced toxicity in retinal endothelial cells. Mol Vis. 2006;12:616–625
  15. Lutty GA, Merges C, Threlkeld AB, Crone S, McLeod DS. Heterogeneity in localization of isoforms of TGF-b in human retina, vitreous, and choroid. Invest Ophthalmol Vis Sci. 1993;34:477–487
  16. Mao HQ, Roy K, Troung-Le VL, Janes KA, Lin KY, Wang Y, et al. Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. J Control Release. 2001;70:399–421
  17. Bhutto IA, Kim SY, McLeod DS, Merges C, Fukai N, Olsen BR, et al. Localization of collagen XVIII and the endostatin portion of collagen XVIII in aged human control eyes and eyes with age-related macular degeneration. Invest Ophthalmol Vis Sci. 2004;45:1544–1552

 This work was supported by the National Eye Institute grant R03EY013744 (G.L.), R01EY09357 (G.L.), EY01765 (Wilmer), the Johns Hopkins Hematology Training grant T32HL007525 (T.P.), the Altsheler Durell Foundation (G.L.), and unrestricted funds from a Research to Prevent Blindness grant (Wilmer).

PII: S1549-9634(08)00086-5

doi: 10.1016/j.nano.2008.06.003

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 4, Issue 4 , Pages 340-349 , December 2008

Article Tools

* Image Usage & Resolution