Volume 5, Issue 2 , Pages 106-117, June 2009
Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles
Abstract
Nanoparticle size and plasma binding profile contribute to a particle's longevity in the bloodstream, which can have important consequences for therapeutic efficacy. In this study an approximate doubling in nanoparticle hydrodynamic size was observed upon in vitro incubation of 30- and 50-nm colloidal gold in human plasma. Plasma proteins that bind the surface of citrate-stabilized gold colloids have been identified. Effects of protein binding on the nanoparticle hydrodynamic size, elements of coagulation, and the complement system have been investigated. The difference in size measurements obtained from dynamic light scattering, electron microscopy, and scanning probe microscopy are also discussed.
Key words: Nanoparticles, Gold colloids, Plasma protein binding, Hydrodynamic size, Complement
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The study was supported in whole or in part by federal funds from the National Cancer Institute, National Institutes of Health, under contract N01-CO-12400.
The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the U.S. government.
PII: S1549-9634(08)00144-5
doi:10.1016/j.nano.2008.08.001
© 2009 Elsevier Inc. All rights reserved.
Volume 5, Issue 2 , Pages 106-117, June 2009
