Nanomedicine: Nanotechnology, Biology and Medicine
Volume 5, Issue 3 , Pages 352-358 , September 2009

Leishmanicidal Activity and Immobilization of dermaseptin 01 antimicrobial peptides in ultrathin films for nanomedicine applications

  • Maysa F. Zampa, MSc

      Affiliations

    • Laboratório de Análises de Combustível, LAPETRO, Universidade Federal do Piauí, UFPI, Teresina, Brazil
    • Departamento de Química, Centro de Ciências da Natureza, CCN, Universidade Federal do Piauí, UFPI, Teresina, Brazil
    • ,
  • Inês M.S. Araújo, BSc

      Affiliations

    • Laboratório de Análises de Combustível, LAPETRO, Universidade Federal do Piauí, UFPI, Teresina, Brazil
    • Departamento de Química, Centro de Ciências da Natureza, CCN, Universidade Federal do Piauí, UFPI, Teresina, Brazil
    • ,
  • Vladimir Costa, MSc

      Affiliations

    • Laboratório de Pesquisas em Leishmanioses, Instituto de Doenças Tropicais Natan Portela–IDTNP, Teresina, Brazil
    • ,
  • Carlos H. Nery Costa, MD, PhD

      Affiliations

    • Laboratório de Pesquisas em Leishmanioses, Instituto de Doenças Tropicais Natan Portela–IDTNP, Teresina, Brazil
    • ,
  • José Ribeiro Santos Jr., PhD

      Affiliations

    • Departamento de Química, Centro de Ciências da Natureza, CCN, Universidade Federal do Piauí, UFPI, Teresina, Brazil
    • ,
  • Valtencir Zucolotto, PhD

      Affiliations

    • Instituto de Física de São Carlos, IFSC, Universidade de São Paulo, USP, Brazil
    • ,
  • Carla Eiras, PhD

      Affiliations

    • Laboratório de Análises de Combustível, LAPETRO, Universidade Federal do Piauí, UFPI, Teresina, Brazil
    • Grupo de Biodiversidade e Biotecnologia, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaiba, Brazil
    • ,
  • José Roberto S.A. Leite, PhD

      Affiliations

    • Laboratório de Análises de Combustível, LAPETRO, Universidade Federal do Piauí, UFPI, Teresina, Brazil
    • Grupo de Biodiversidade e Biotecnologia, Campus Ministro Reis Velloso, CMRV, Universidade Federal do Piauí, UFPI, Parnaiba, Brazil
    • Corresponding Author InformationCorresponding author. Campus Ministro Reis Velloso (CMRV), Universidade Federal do Piauí (UFPI), Rua Pinheiro Machado Reis Velloso 64200200–Parnaiba, PI, Brazil.

Received 7 August 2008 ,Accepted 3 November 2008.

References 

  1. Borg MA. Bed occupancy and overcrowding as determinant factors in the incidence of MRSA infections within general ward settings. J Hosp Infect. 2003;54:316–318
  2. Kubo I, Fujita KI, Nihei KI. Molecular design of multifunctional antibacterial agents against methicillin resistant Staphylococcus aureus (MRSA). Bioorg Med Chem Lett. 2003;11:4255–4262
  3. Zhang L, Falla TJ. Antimicrobial peptides: therapeutic potential. Exp Opin Pharmacother. 2006;7:653–663
  4. Brand GD, Leite JRSA, Sá Mendel SM, Mesquita DA, Silva LP, Prates MV, et al. Novel dermaseptins from Phyllomedusa hypochondrialis (Amphibia). Biochem Biophys Res Commun. 2006;347:739–746
  5. Leite JRSA, Brand GD, Silva LP, Kückelhaus SAS, Bento WRC, Araújo ALT, et al. Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta: secondary structure, antimicrobial activity, and mammalian cell toxicity. Comp Biochem Physiol A Mol Integr Physiol. 2008;151:336–343
  6. Chinchar VG, Bryan L, Silphadaung U, Noga E, Wade D, Rollins-Smith L. Inactivation of viruses infecting ectothermic animals by amphibian and piscine antimicrobial peptides. Virology. 2004;323:268–275
  7. Lorin C, Saidi H, Belaid A, Zairi A, Baleux F, Hocini H, et al. The antimicrobial peptide dermaseptin S4 inhibits HIV-1 infectivity in vitro. Virology. 2005;334:264–275
  8. Mor A, Hani K, Nicolas P. The vertebrate peptide antibiotics dermaseptins have overlapping structural features but target specific microorganisms. J Biol Chem. 1994;269:31635–31641
  9. Brand GD, Leite JRSA, Silva LP, Albuquerque S, Prates MV, Azevedo RB, et al. Dermaseptins from Phyllomedusa oreades and Phyllomedusa distincta: anti-Trypanosoma cruzi activity without cytotoxicity to mammalian cells. J Biol Chem. 2002;277:49332–49340
  10. De Lucca AJ, Bland JM, Jacks TJ, Grimm C, Walsh TJ. Fungicidal and binding properties of the natural peptides cecropin B and dermaseptin. Med Mycol. 1998;36:291–298
  11. Efron L, Dagan A, Gaidukov L, Ginsburg H, Mor A. Direct interaction of dermaseptin S4 aminoheptanoyl derivative with intraerythrocytic malaria parasite leading to increased specific antiparasitic activity in culture. J Biol Chem. 2002;277:24067–24072
  12. Brand GD, Krause FC, Silva LP, Leite JRSA, Melo JA, Prates MV, et al. Bradykinin-related peptides from Phyllomedusa hypochondrialis. Peptides. 2006;27:2137–2146
  13. Hancock REW, Lehrer R. Cationic peptides: a new source of antibiotics. Trends Biotechnol. 1998;16:82–87
  14. Bracci L, Falciani C, Lelli B, Lozzi L, Runci Y, Pini A, et al. Synthetic peptides in the form of dendrimers become resistant to protease activity. J Biol Chem. 2003;278:46590–46595
  15. Tam PJ, Lu YA, Yang JL. Antimicrobial dendrimeric peptides. Eur J Biochem. 2002;269:923–932
  16. Pini A, Giuliani A, Falciani C, Runci Y, Ricci C, Lelli B, et al. Antimicrobial activity of novel dendrimeric peptides obtained by phage display selection and rational modification. Antimicrob Agents Chemother. 2005;49:2665–2672
  17. Huguenin F, Zucolotto V, Carvalho AJF, Gonzalez ER, Oliveira ON. Layer-by-layer hybrid films incorporating WO3, TiO2 and chitosan. Chem Mater. 2005;17:6739–6745
  18. Zucolotto V, Pinto APA, Tumolo T, Moraes ML, Baptista MS, Riul A, et al. Catechol biosensing using a nanostructured layer-by-layer film containing Cl-catechol 1,2 dioxygenase. Biosensors Bioelectronics. 2006;21:1320–1326
  19. Zucolotto V, Daghastanli KRP, Hayasaka CO, Riul A, Ciancaglini P, Oliveira ON. Using capacitance measurements as the detection method in antigen-containing layer-by-layer films for biosensing. Anal Chem. 2007;79:2163–2167
  20. Zampa MF, Brito ACF, Kitagawa IL, Constantino CJL, Oliveira ON, Cunha HN, et al. Natural gum-assisted phthalocyanine immobilization in electroactive nanocomposites: physicochemical characterization and sensing applications. Biomacromolecules. 2007;8:3408–3413
  21. Siqueira JR, Gasparotto LHS, Crespilho FN, Carvalho AJF, Zucolotto V, Oliveira ON. Physicochemical properties and sensing ability of metallophthalocyanines/chitosan nanocomposites. J Phys Chem B. 2006;110:22690–22694
  22. Savoia D, Avanzini C, Conti S, Magliani W, Frazzi R, Polonelli L. In vitro leishmanicidal activity of a monoclonal antibody mimicking a yeast killer toxin. J Eukaryot Microbiol. 2002;49:319–323
  23. Desjeux P, Alvar J. Leishmania/HIV co-infections: epidemiology in Europe. Ann Trop Med Parasitology. 2003;97:3–15
  24. Marty P, Le Fichoux Y, Pratlong F, Gari-Toussaint M. Human visceral leishmaniasis in Alpes-Maritimes, France: epidemiological characteristics for the period 1985–1992. Trans R Soc Trop Med Hyg. 1994;88:33–34
  25. Bates PA, Robertson CD, Tetley L, Coombs GH. Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms. Parasitology. 1992;105:193–202
  26. Tomás AM, Kelly JM. Stage-regulated expression of cruzipain, the major cysteine-proteinase of Trypanosoma cruzi is independent of the level of RNA. Mol Biochem Parasitol. 1996;76:91–104
  27. Shukla AK, Paliteiro C, Manoharan R, Hamnett A, Goodenough JB. Efficient oxygen reduction in alkaline solution with platinum phthalocyanine on porous carbon. J Appl Electrochem. 1899;19:105
  28. Löfgren SE, Miletti LC, Steindel M, Bachère E, Barracco MA. Trypanocidal and leishmanicidal activities of different antimicrobial peptides (AMPs) isolated from aquatic animals. Exp Parasitol. 2008;118:197–202
  29. Chicharro C, Granata C, Lozano R, Andreu D, Rivas L. N-terminal fatty acid substitution increases the leishmanicidal activity of CA(1–7)M(2–9), a cecropin–melittin hybrid peptide. Antimicrob Agents Chemother. 2001;45:2441–2449
  30. Feder R, Dagan A, Mor A. Structure–activity relationship study of antimicrobial dermaseptin S4 showing the consequences of peptide oligomerization on selective cytotoxicity. J Biol Chem. 2000;275:4230–4238
  31. Silva PI, Daffre S, Bulet P. Isolation and characterization of gomesin, an 18-residue cysteine-rich defense peptide from the spider Acanthoscurria gomesiana hemocytes with sequence similarities to horseshoe crab antimicrobial peptides of the tachyplesin family. J Biol Chem. 2000;275:33464–33470
  32. Mangoni ML, Saugar JM, Dellisanti M, Barra D, Simmaco M, Rivas L. Temporins, small antimicrobial peptides with leishmanicidal activity. J Biol Chem. 2005;280:984–990
  33. Barr SC, Rose D, Jaynes JM. Activity of lytic peptides against intracellular Trypanosoma cruzi amastigotes in vitro and parasitemias in mice. J Parasitol. 1995;81:974–978
  34. Leite JRSA, Silva LP, Rodrigues MIS, Prates MV, Brand GD, Lacava BM, et al. Phylloseptins: a novel class of anti-bacterial and anti-protozoan peptides from the Phyllomedusa genus. Peptides. 2005;26:565–573
  35. Allan IJ, Vrana B, Greenwood R, Mills GA, Roig B, Gonzalez C. A 'toolbox' for chemical and biological monitoring requirements for the European Union's Water Framework Directive. Talanta. 2006;69:302–322
  36. Rick J, Chou TC. Amperometric protein sensor—fabricated as a polypyrrole, poly-aminophenylboronic acid bilayer. Biosensors Bioelectronics. 2006;22:329–335
  37. Dahint R, Trileva E, Acunmana H, Konrad U, Zimmera M, Stadler V, et al. Optically responsive nanoparticle layers for the label-free analysis of biospecific interactions in array formats. Biosensors Bioelectronics. 2007;22:3174–3181
  38. Mehta M, Hanumanthaiah CS, Betala PA, Zhang H, Roh SW, Buttner W, et al. Detection of proteins and bacteria using an array of feedback capacitance sensors. Biosensors Bioelectronics. 2007;23:728–734
  39. Fowler JM, Stuart MC, Wong DKY. An amperometric immunosensor with a thiolated Protein G scaffold. Electrochem Commun. 2008;10:1020–1023
  40. Kafi AK, Lee DY, Park SH, Kwon YS. A hydrogen peroxide biosensor based on peroxidase activity of hemoglobin in polymeric film. J Nanosci Nanotechnol. 2007;7:4005–4008

 The study was supported entirely by FAPEPI (PPP/2006 – Programa Primeiros Projetos and Programa Fluxo Contínuo/2008), FAPESP, CAPES, CNPq, and IMMP/MCT for financial support. This work was partially financed by grants from LAPETRO and UFPI (Universidade Federal do Piauí). Neither commercial association current or within the past five years nor any conflict of interest exists.

PII: S1549-9634(08)00187-1

doi: 10.1016/j.nano.2008.11.001

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 5, Issue 3 , Pages 352-358 , September 2009

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