Nanomedicine: Nanotechnology, Biology and Medicine
Volume 5, Issue 4 , Pages 402-409 , December 2009

Sensitive and multiplexed detection of proteomic antigens via quantum dot aggregation

  • Chinmay Soman, BE

      Affiliations

    • Interdisciplinary Program in Materials Science, Vanderbilt University, Nashville, Tennessee, USA
    • ,
  • Todd Giorgio, PhD

      Affiliations

    • Biomedical Engineering, Vanderbilt University, Nashville, Tennessee, USA
    • Corresponding Author InformationCorresponding author: Biomedical Engineering, Vanderbilt University, Box 351631 B, Nashville, TN 37235, USA.

Received 6 August 2008 ,Accepted 16 January 2009.

References 

  1. Arntz Y, Seelig JD, Lang HP, Zhang J, Hunziker P, Ramseyer JP, et al. Label-free protein assay based on a nanomechanical cantilever array. Nanotechnology. 2003;14:86–90
  2. Salata OV. Applications of nanoparticles in biology and medicine. J Nanobiotechnol. 2004;2:3–8
  3. Wang J. Carbon-nanotube based electrochemical biosensors: a review. Electroanalysis. 2005;17:7–14
  4. Ferrari M. Cancer nanotechnology: opportunities and challenges. Nat Rev Cancer. 2005;5:161–171
  5. Soman CP, Giorgio TD. Quantum dot self-assembly for protein detection with sub-picomolar sensitivity. Langmuir. 2008;24:4399–4404
  6. Goldman ER, Balighian ED, Mattoussi H, Kuno MK, Mauro JM, Tran PT, et al. Avidin: a natural bridge for quantum dot–antibody conjugates. J Am Chem Soc. 2002;124:6378–6382
  7. Plotz CM, Singer JM. The latex fixation test. I. Application to the serologic diagnosis of rheumatoid arthritis. Am J Med. 1956;21:888–892
  8. March SB, Ratnam S. Latex agglutination test for detection of Escherichia coli serotype O157. J Clin Microbiol. 1989;27:1675–1677
  9. van Griethuysen A, Pouw M, van Leeuwen N, Heck M, Willemse P, Buiting A, et al. Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus. J Clin Microbiol. 1999;37:2789–2792
  10. Sulahian A, Tabouret M, Ribaud P, Sarfati J, Gluckman E, Latgé JP, et al. Comparison of an enzyme immunoassay and latex agglutination test for detection of galactomannan in the diagnosis of invasive aspergillosis. Eur J Clin Microbiol Infect Dis. 1996;15:139–145
  11. Storhoff JJ, Elghanian R, Mucic RC, Mirkin CA, Letsinger RL. One-pot colorimetric differentiation of polynucleotides with single base imperfections using gold nanoparticle probes. J Am Chem Soc. 1998;120:1959–1964
  12. Hirsch LR, Jackson JB, Lee A, Halas NJ, West JL. A whole blood immunoassay using gold nanoshells. Anal Chem. 2003;75:2377–2381
  13. Ho YP, Kung MC, Yang S, Wang TH. Multiplexed hybridization detection with multicolor colocalization of quantum dot nanoprobes. Nano Lett. 2005;5:1693–1697
  14. Agrawal A, Tripp RA, Anderson LJ, Nie S. Real-time detection of virus particles and viral protein expression with two-color nanoparticle probes. J Virol. 2005;79:8625–8628
  15. Camilla C, Mely L, Magnan A, Casano B, Prato S, Debono S, et al. Flow cytometric microsphere-based immunoassay: analysis of secreted cytokines in whole-blood samples from asthmatics. Clin Vaccine Immunol. 2001;8:776–784
  16. Kellar KL, Iannone MA. Multiplexed microsphere-based flow cytometric assays. Exp Hematol. 2002;30:1227–1237
  17. Calvo KR, Liotta LA, Petricoin EF. Clinical proteomics: from biomarker discovery and cell signaling profiles to individualized personal therapy. Biosci Rep. 2005;25:107–125
  18. Petricoin EF, Liotta LA. Proteomic approaches in cancer risk and response assessment. Trends Mol Med. 2004;10:59–64
  19. Roboz J. Mass spectrometry in diagnostic oncoproteomics. Cancer Invest. 2005;23:465–478
  20. Polanski M, Anderson NL. A list of candidate cancer biomarkers for targeted proteomics. Biomarker Insights. 2006;2:1–48
  21. Miyashita M, Tajiri T, Yanagi K, Shimizu T, Futami R, Sasajima K, et al. Serum levels of vascular endothelial growth factor, basic fibroblast growth factor and endostatin in human metastatic liver tumors. Hepatogastroenterology. 2003;50:308–309
  22. Lobov IB, Brooks PC, Lang RA. Angiopoietin-2 displays VEGF-dependent modulation of capillary structure and endothelial cell survival in vivo. Proc Natl Acad Sci U S A. 2002;99:11205–11210
  23. Maisonpierre PC, Suri C, Jones PF, Bartunkova S, Wiegand SJ, Radziejewski C, et al. Angiopoietin-2, a natural antagonist for Tie2 that disrupts in vivo angiogenesis. Science. 1997;277:55–60
  24. Carmeliet P, Jain RK. Angiogenesis in cancer and other diseases. Nature. 2000;407:249–257
  25. Polanski M, Anderson NL. A list of candidate cancer biomarkers for targeted proteomics. Biomarker Insights. 2006;2:1–48
  26. Audero E, Arisio R, Bussolino F, Sismondi P, De Bortoli M. Angiopoietin-2 expression in breast cancer correlates with lymph node invasion and short survival. Int J Cancer. 2003;103:466–474
  27. Caine GJ, Blann AD, Stonelake PS, Ryan P, Lip GYH. Plasma angiopoietin-1, angiopoietin-2 and Tie-2 in breast and prostate cancer: a comparison with VEGF and Flt-1. Eur J Clin Invest. 2003;33:883–890
  28. Talapin DV, Rogach AL, Kornowski A, Haase M, Weller H. Highly luminescent monodisperse CdSe and CdSe /ZnS nanocrystals synthesized in a hexadecylamine-trioctylphosphine oxide–trioctylphospine mixture. Nano Lett. 2001;1:207–211
  29. Hines MA, Guyot-Sionnest P. Synthesis and characterization of strongly luminescing ZnS-capped CdSe nanocrystals. J Phys Chem. 1996;100:468–471
  30. Ebenstein Y, Mokari T, Banin U. Fluorescence quantum yield of CdSe/ZnS nanocrystals investigated by correlated atomic-force and single-particle fluorescence microscopy. Appl Phys Lett. 2002;80:4033–4035
  31. Underwood DF, Kippeny T, Rosenthal SJ. Ultrafast carrier dynamics in CdSe nanocrystals determined by femtosecond fluorescence upconversion spectroscopy. J Phys Chem. 2001;436–443
  32. Bruchez M. Semiconductor nanocrystals as fluorescent biological labels. Science. 1998;281:2013–2016
  33. Dubertret B. In vivo imaging of quantum dots encapsulated in phospholipid micelles. Science. 2002;298:1759–1762
  34. Chan WCW, Maxwell DJ, Gao X, Bailey RE, Han M, Nie S. Luminescent quantum dots for multiplexed biological detection and imaging. Curr Opin Biotechnol. 2002;13:40–46
  35. Xu H. Multiplexed SNP genotyping using the qbead system: a quantum dot-encoded microsphere-based assay. Nucleic Acids Res. 2003;31:43
  36. Gao X, Chan WCW, Nie S. Quantum-dot nanocrystals for ultrasensitive biological labeling and multicolor optical encoding. J Biomed Opt. 2002;7:532–537
  37. Goldman ER, Medintz IL, Mattoussi H. Luminescent quantum dots in immunoassays. Anal Bioanal Chem. 2006;384:560–563
  38. Klostranec JM, Chan WCW. Quantum dots in biological and biomedical research: recent progress and present challenges. Adv Mater. 2006;18:1953–1964
  39. Ott L. An introduction to statistical methods and data analysis. North Scituate. Massachusetts: Duxbury Press; 1993;
  40. Soman CP, Giorgio TD. Kinetics of molecular recognition mediated nanoparticle self-assembly. Nano Res. 2009;2:78–84

 No conflict of interest was reported by the authors of this paper.

PII: S1549-9634(09)00048-3

doi: 10.1016/j.nano.2009.01.010

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 5, Issue 4 , Pages 402-409 , December 2009

Article Tools

* Image Usage & Resolution