Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood–derived mesenchymal stem cells

Park, Ki-Soo; Tae, Jinsung; Choi, Bongkum; Kim, Young-Seok; Moon, Cheol; Kim, Sa-Hyun; Lee, Han-Sin; Kim, Jinhyun; Kim, Junsung; Park, Jaeberm; Lee, Jung-Hee; Lee, Jong Eun; Joh, Jae-Won; Kim, Sungjoo

doi:10.1016/j.nano.2009.07.005

« Previous Next »Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 2 , Pages 263-276, April 2010

Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood–derived mesenchymal stem cells

Ki-Soo Park, DVM, MS
- Department of Molecular Medicine, Sungkyunkwan University, Seoul, South Korea
- Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
Jinsung Tae, PhD
- Department of Chemistry, Yonsei University School of Chemistry, Seoul, South Korea
Bongkum Choi, MS
- Department of Molecular Medicine, Sungkyunkwan University, Seoul, South Korea
- Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
Young-Seok Kim, MS
- Department of Molecular Medicine, Sungkyunkwan University, Seoul, South Korea
Cheol Moon, PhD
- Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
- Department of Clinical Laboratory Science, 579, Sinwol-Dong, Jecheon, 390-711 Chungbuk, South Korea
Sa-Hyun Kim, MS
- Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
Han-Sin Lee, MS
- Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
Jinhyun Kim, DVM
- Bio-material Science Laboratory, BITERIALS Co. Ltd., Seoul, South Korea
Junsung Kim, DVM, PhD
- Bio-material Science Laboratory, BITERIALS Co. Ltd., Seoul, South Korea
Jaeberm Park, MD, MS
- Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
Jung-Hee Lee, PhD
- Department of Radiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
Jong Eun Lee, PhD
- Department of Anatomy, BK21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea
Jae-Won Joh, MD, PhD
- Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
Sungjoo Kim, MD, PhD
- Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
- Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
- Corresponding author: Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, South Korea.

Received 7 January 2009; accepted 16 July 2009. published online 21 August 2009.

Abstract

Live imaging is a powerful technique that can be used to characterize the fate and location of stem cells in animal models. Here we investigated the characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, MNPs@SiO₂(RITC). We also conducted various in vivo–uptake tests with nanoparticle-labeled human MSCs. MNPs@SiO₂(RITC) showed photostability against ultraviolet light exposure and were nontoxic to human MSCs, based on the MTT, apoptosis, and cell cycle arrest assays. In addition, MNPs@SiO₂(RITC) did not affect the surface phenotype or morphology of human MSCs. We also demonstrated that MNPs@SiO₂(RITC) have stable retention properties in MSCs in vitro. Furthermore, using optical and magnetic resonance imaging, we successfully detected a visible signal from labeled human MSCs that were transplanted into NOD.CB17-Prkdc^SCID (NOD-SCID) mice. These results demonstrate that MNPs@SiO₂(RITC) are biocompatible and useful tools for human MSC labeling and bioimaging.

From the Clinical Editor

The characteristics and in vitro cytotoxicity of human mesenchymal stem cells (MSCs) labeled with silica-coated magnetic nanoparticles incorporating rhodamine B isothiocyanate, RITC were investigated in this study. RITC showed photostability against ultraviolet light exposure and was nontoxic to human MSCs. Using both optical and magnetic resonance imaging, successful detection of signal from labeled human MSCs transplanted into mice is demonstrated.

Key words: Biocompatibility, Mesenchymal stem cells, Magnetic resonance imaging, Nanoparticles, Stem cells