Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 2 , Pages 263-276 , April 2010

Characterization, in vitro cytotoxicity assessment, and in vivo visualization of multimodal, RITC-labeled, silica-coated magnetic nanoparticles for labeling human cord blood–derived mesenchymal stem cells

  • Ki-Soo Park, DVM, MS

      Affiliations

    • Department of Molecular Medicine, Sungkyunkwan University, Seoul, South Korea
    • Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
  • ,
  • Jinsung Tae, PhD

      Affiliations

    • Department of Chemistry, Yonsei University School of Chemistry, Seoul, South Korea
  • ,
  • Bongkum Choi, MS

      Affiliations

    • Department of Molecular Medicine, Sungkyunkwan University, Seoul, South Korea
    • Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
  • ,
  • Young-Seok Kim, MS

      Affiliations

    • Department of Molecular Medicine, Sungkyunkwan University, Seoul, South Korea
  • ,
  • Cheol Moon, PhD

      Affiliations

    • Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
    • Department of Clinical Laboratory Science, 579, Sinwol-Dong, Jecheon, 390-711 Chungbuk, South Korea
  • ,
  • Sa-Hyun Kim, MS

      Affiliations

    • Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
  • ,
  • Han-Sin Lee, MS

      Affiliations

    • Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
  • ,
  • Jinhyun Kim, DVM

      Affiliations

    • Bio-material Science Laboratory, BITERIALS Co. Ltd., Seoul, South Korea
  • ,
  • Junsung Kim, DVM, PhD

      Affiliations

    • Bio-material Science Laboratory, BITERIALS Co. Ltd., Seoul, South Korea
  • ,
  • Jaeberm Park, MD, MS

      Affiliations

    • Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
  • ,
  • Jung-Hee Lee, PhD

      Affiliations

    • Department of Radiology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
  • ,
  • Jong Eun Lee, PhD

      Affiliations

    • Department of Anatomy, BK21 Project for Medical Science, Yonsei University College of Medicine, Seoul, South Korea
  • ,
  • Jae-Won Joh, MD, PhD

      Affiliations

    • Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
  • ,
  • Sungjoo Kim, MD, PhD

      Affiliations

    • Transplantation Research Center, Samsung Biomedical Research Institute, Seoul, South Korea
    • Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, South Korea
    • Corresponding Author InformationCorresponding author: Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, South Korea.

Received 7 January 2009 ,Accepted 16 July 2009.

References 

  1. Bicknese AR, Goodwin HS, Quinn CO, Henderson VC, Chien SN, Wall DA. Human umbilical cord blood cells can be induced to express markers for neurons and glia. Cell Transplant. 2002;11:261–264
  2. Deans RJ, Moseley AB. Mesenchymal stem cells: biology and potential clinical uses. Exp Hematol. 2000;28:875–884
  3. Erices A, Conget P, Minguell JJ. Mesenchymal progenitor cells in human umbilical cord blood. Br J Haematol. 2000;109:235–242
  4. Karnieli O, Izhar-Prato Y, Bulvik S, Efrat S. Generation of insulin-producing cells from human bone marrow mesenchymal stem cells by genetic manipulation. Stem Cells. 2007;25:2837–2844
  5. Timper K, Seboek D, Eberhardt M, Linscheid P, Christ-Crain M, Keller U, et al. Human adipose tissue-derived mesenchymal stem cells differentiate into insulin, somatostatin, and glucagon expressing cells. Biochem Biophys Res Commun. 2006;341:1135–1140
  6. Rabin N, Kyriakou C, Coulton L, Gallagher OM, Buckle C, Benjamin R, et al. A new xenograft model of myeloma bone disease demonstrating the efficacy of human mesenchymal stem cells expressing osteoprotegerin by lentiviral gene transfer. Leukemia. 2007;21:2181–2191
  7. Sun L, Cui M, Wang Z, Feng X, Mao J, Chen P, et al. Mesenchymal stem cells modified with angiopoietin-1 improve remodeling in a rat model of acute myocardial infarction. Biochem Biophys Res Commun. 2007;357:779–784
  8. Noiseux N, Gnecchi M, Lopez-Ilasaca M, Zhang L, Solomon SD, Deb A, et al. Mesenchymal stem cells overexpressing Akt dramatically repair infarcted myocardium and improve cardiac function despite infrequent cellular fusion or differentiation. Mol Ther. 2006;14:840–850
  9. Meinel L, Hofmann S, Betz O, Fajardo R, Merkle HP, Langer R, et al. Osteogenesis by human mesenchymal stem cells cultured on silk biomaterials: comparison of adenovirus mediated gene transfer and protein delivery of BMP-2. Biomaterials. 2006;27:4993–5002
  10. Le Blanc K, Ringden O. Immunobiology of human mesenchymal stem cells and future use in hematopoietic stem cell transplantation. Biol Blood Marrow Transplant. 2005;11:321–334
  11. Aggarwal S, Pittenger MF. Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood. 2005;105:1815–1822
  12. Lv H, Zhang S, Wang B, Cui S, Yan J. Toxicity of cationic lipids and cationic polymers in gene delivery. J Control Release. 2006;114:100–109
  13. Pleyer U, Groth D, Hinz B, Keil O, Bertelmann E, Rieck P, et al. Efficiency and toxicity of liposome-mediated gene transfer to corneal endothelial cells. Exp Eye Res. 2001;73:1–7
  14. Howard DB, Powers K, Wang Y, Harvey BK. Tropism and toxicity of adeno-associated viral vector serotypes 1, 2, 5, 6, 7, 8, and 9 in rat neurons and glia in vitro. Virology. 2008;372:24–34
  15. Banin E, Obolensky A, Piontek E, Falk H, Pikarsky E, Pe'er J, et al. Gene delivery by viral vectors in primary cultures of lacrimal gland tissue. Invest Ophthalmol Vis Sci. 2003;44:1529–1533
  16. Stone D, David A, Bolognani F, Lowenstein PR, Castro MG. Viral vectors for gene delivery and gene therapy within the endocrine system. J Endocrinol. 2000;164:103–118
  17. Greenbaum L, Rothmann C, Lavie R, Malik Z. Green fluorescent protein photobleaching: a model for protein damage by endogenous and exogenous singlet oxygen. Biol Chem. 2000;381:1251–1258
  18. Chen TS, Zeng SQ, Luo QM, Zhang ZH, Zhou W. High-order photobleaching of green fluorescent protein inside live cells in two-photon excitation microscopy. Biochem Biophys Res Commun. 2002;291:1272–1275
  19. Giloh H, Sedat JW. Fluorescence microscopy: reduced photobleaching of rhodamine and fluorescein protein conjugates by n-propyl gallate. Science. 1982;217:1252–1255
  20. Dittel BN, Visintin I, Merchant RM, Janeway CA. Presentation of the self antigen myelin basic protein by dendritic cells leads to experimental autoimmune encephalomyelitis. J Immunol. 1999;163:32–39
  21. Shah BS, Clark PA, Moioli EK, Stroscio MA, Mao JJ. Labeling of mesenchymal stem cells by bioconjugated quantum dots. Nano Lett. 2007;7:3071–3079
  22. Lu CW, Hung Y, Hsiao JK, Yao M, Chung TH, Lin YS, et al. Bifunctional magnetic silica nanoparticles for highly efficient human stem cell labeling. Nano Lett. 2007;7:149–154
  23. Muller-Borer BJ, Collins MC, Gunst PR, Cascio WE, Kypson AP. Quantum dot labeling of mesenchymal stem cells. J Nanobiotechnol. 2007;5:9
  24. He G, Zhang H, Wei H, Wang Y, Zhang X, Tang Y, et al. In vivo imaging of bone marrow mesenchymal stem cells transplanted into myocardium using magnetic resonance imaging: a novel method to trace the transplanted cells. Int J Cardiol. 2007;114:4–10
  25. Weis K. Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle. Cell. 2003;112:441–451
  26. Lovric J, Bazzi HS, Cuie Y, Fortin GR, Winnik FM, Maysinger D. Differences in subcellular distribution and toxicity of green and red emitting CdTe quantum dots. J Mol Med. 2005;83:377–385
  27. Sadler PJ, Viles JH. 1H and 113Cd NMR investigations of Cd2+ and Zn2+ binding sites on serum albumin: competition with Ca2+, Ni2+, Cu2+, and Zn2+. Inorg Chem. 1996;35:4490–4496
  28. Yoon TJ, Kim JS, Kim BG, Yu KN, Cho MH, Lee JK. Multifunctional nanoparticles possessing a “magnetic motor effect” for drug or gene delivery. Angew Chem Int Ed Engl. 2005;44:1068–1071
  29. Zins D, Cabuil V, Massart R. New aqueous magnetic fluids. J Mol Liq. 1999;83:217–232
  30. Sousa MH, Tourinho FA, Depeyrot J, da Silva GJ, Lara MCFL. New electric double-layered magnetic fluids based on copper, nickel, and zinc ferrite nanostructures. J Phys Chem B. 2001;105:1168–1175
  31. Lu Y, Yin Y, Mayers BT, Xia Y. Modifying the surface properties of superparamagnetic iron oxide nanoparticles through a sol-gel approach. Nano Lett. 2002;2:183–186
  32. Bieback K, Kern S, Kluter H, Eichler H. Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood. Stem Cells. 2004;22:625–634
  33. Verhaegh NA, van Blaaderen A. Dispersions of rhodamine-labeled silica spheres—synthesis, characterization, and fluorescence confocal scanning laser microscopy. Langmuir. 1994;10:1427–1438
  34. Kim JS, Yoon TJ, Yu KN, Kim BG, Park SJ, Kim HW, et al. Toxicity and tissue distribution of magnetic nanoparticles in mice. Toxicol Sci. 2006;89:338–347
  35. Yoon TJ, Yu KN, Kim E, Kim JS, Kim BG, Yun SH, et al. Specific targeting, cell sorting, and bioimaging with smart magnetic silica core-shell nanomaterials. Small. 2006;2:209–215
  36. Hoshino A, Manabe N, Fujioka K, Suzuki K, Yasuhara M, Yamamoto K. Use of fluorescent quantum dot bioconjugates for cellular imaging of immune cells, cell organelle labeling, and nanomedicine: surface modification regulates biological function, including cytotoxicity. J Artif Organs. 2007;10:149–157
  37. Hoshino A, Hanaki K, Suzuki K, Yamamoto K. Applications of T-lymphoma labeled with fluorescent quantum dots to cell tracing markers in mouse body. Biochem Biophys Res Commun. 2004;314:46–53
  38. Huang DM, Hung Y, Ko BS, Hsu SC, Chen WH, Chien CL, et al. Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking. FASEB J. 2005;19:2014–2016
  39. Song YS, Ku JH. Monitoring transplanted human mesenchymal stem cells in rat and rabbit bladders using molecular magnetic resonance imaging. Neurourol Urodyn. 2007;26:584–593
  40. Shah B, Clark P, Stroscio M, Mao J. Labeling and imaging of human mesenchymal stem cells with quantum dot bioconjugates during proliferation and osteogenic differentiation in long term. Conf Proc IEEE Eng Med Biol Soc. 2006;1:1470–1473
  41. Muller-Borer BJ, Collins MC, Gunst PR, Cascio WE, Kypson AP. Quantum dot labeling of mesenchymal stem cells. J Nanobiotechnol. 2007;5:1–9
  42. Greulich C, Kittler S, Epple M, Muhr G, Köller M. Studies on the biocompatibility and the interaction of silver nanoparticles with human mesenchymal stem cells (hMSCs). Langenbecks Arch Surg. 2009;394:495–502
  43. Huang DM, Hsiao JK, Chen YC, Chien LY, Yao M, Chen YK, et al. The promotion of human mesenchymal stem cell proliferation by superparamagnetic iron oxide nanoparticles. Biomaterials. 2009;30:3645–3651
  44. Amsalem Y, Mardor Y, Feinberg MS, Landa N, Miller L, Daniels D, et al. Iron-oxide labeling and outcome of transplanted mesenchymal stem cells in the infarcted myocardium. Circulation. 2007;116:138–145
  45. Lin S, Xie X, Patel MR, Yang YH, Li Z, Cao F, et al. Quantum dot imaging for embryonic stem cells. BMC Biotechnol. 2007;7:67–76
  46. Guzman R, Uchida N, Bliss TM, He D, Christopherson KK, Stellwagen D, et al. Long-term monitoring of transplanted human neural stem cells in developmental and pathological contexts with MRI. Proc Natl Acad Sci U S A. 2007;104:10211–10216
  47. Tai JH, Foster P, Rosales A, Feng B, Hasilo C, Martinez V, et al. Imaging islets labeled with magnetic nanoparticles at 1.5 Tesla. Diabetes. 2006;55:2931–2938
  48. Shapiro EM, Sharer K, Skrtic S, Koretsky AP. In vivo detection of single cells by MRI. Magn Reson Med. 2006;55:242–249
  49. Cheon J, Lee JH. Synergistically integrated nanoparticles as multimodal probes for nanobiotechnology. Acc Chem Res. 2008;41:1630–1640

 This research was supported by grants from the IN-SUNG Foundation for Medical Research, Seoul, South Korea.

PII: S1549-9634(09)00166-X

doi: 10.1016/j.nano.2009.07.005

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 2 , Pages 263-276 , April 2010