Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 3 , Pages 463-470 , June 2010

Dendrosome-based delivery of siRNA against E6 and E7 oncogenes in cervical cancer

  • Tathagata Dutta, PhD

      Affiliations

    • School of Pharmacy, University of Queensland, Brisbane, Australia
    • Diamantina Institute for Cancer, Immunology and Metabolic Medicine, University of Queensland, Brisbane, Australia
    • ,
  • Melinda Burgess, MSc

      Affiliations

    • Diamantina Institute for Cancer, Immunology and Metabolic Medicine, University of Queensland, Brisbane, Australia
    • ,
  • Nigel A.J. McMillan, PhD

      Affiliations

    • Diamantina Institute for Cancer, Immunology and Metabolic Medicine, University of Queensland, Brisbane, Australia
    • ,
  • Harendra S. Parekh, PhD

      Affiliations

    • School of Pharmacy, University of Queensland, Brisbane, Australia
    • Corresponding Author InformationCorresponding author: School of Pharmacy, University of Queensland, St Lucia, Brisbane, QLD4072, Australia.

Received 3 June 2009 ,Accepted 2 December 2009.

References 

  1. Franco EL, Schlecht NF, Saslow D. The epidemiology of cervical cancer. Cancer. 2006;9:348–359
  2. DiPaolo JA, Alvarez-Salas LM. Advances in the development of therapeutic nucleic acids against cervical cancer. Expert Opin Biol Ther. 2004;4:1251–1264
  3. Parkin DM, Bray FI, Devesa SS. Cancer burden in the year 2000, The global picture. Eur. J Cancer. 2001;37:S4–S66
  4. Parkin DM, Bray F, Ferlay J, Pisani P. Global cancer statistics. CA Cancer J Clin. 2002;55:74–108
  5. Mantovani F, Banks L. The human papillomavirus E6 protein and its contribution to malignant progression. Oncogene. 2001;20:7874–7887
  6. Schniffner M, Whitaker NJ. Human papillomavirus induced carcinogenesis and the ubiquitin-proteasome system. Semin Cancer Biol. 2003;13:59–67
  7. Lopez-Ocejo O, Viloria-Petit A, Bequet-Romero M, Mukhopadhyay D, Rak J, Kerbel RS. Oncogenes and tumor angiogenesis: the HPV-16 E6 oncoprotein activates the vascular endothelial growth factor (VEGF) gene promoter in a p53 independent manner. Oncogene. 2000;19:4611–4620
  8. Bequet-Romero M, Lopez-Ocejo O. Angiogenesis modulators expression in culture cell lines positives for HPV-16 oncoproteins. Biochem. Biophys. Res. Commun. 2000;277:55–61
  9. Toussaint-Smith E, Donner DB, Roman A. Papillomavirus type 16 E6 and E7 oncoproteins in primary foreskin keratinocytes is sufficient to alter the expression of angiogenic factors. Oncogene. 2004;23:2988–2995
  10. Dasgupta R, Perrimon N. Using RNAi to catch Drosophila genes in a web of interactions: insights into cancer research. Oncogene. 2004;23:8359–8365
  11. Jiang M, Milner J. Selective silencing of viral gene expression in HPV positive human cervical carcinoma cells treated with siRNA, a primer of RNA interference. Oncogene. 2002;21:6041–6048
  12. Yoshinouchi M, Yamada T, Kizaki M, Fen J, Koseki T, Ikeda Y. In vitro and in vivo growth suppression of human papillomavirus 16-positive cervical cancer cells by E6 siRNA. Mol Ther. 2003;8:762–768
  13. Yamato K, Fen J, Kobuchi H, Nasu Y, Yamada T, Nishihara T, et al. Induction of cell death in human papillomavirus 18-positive cervical cancer cells by E6 siRNA. Cancer Gene Ther. 2006;13:234–241
  14. Butz K, Ristriani T, Hengstermann A, Denk C, Scheffner M, Hoppe- Seyler F. siRNA targeting of the viral E6 oncogene efficiently kills human papillomavirus-positive cancer cells. Oncogene. 2003;22:5938–5945
  15. Hall AH, Alexander KA. RNA interference of human papillomavirus type 18 E6 and E7 induces senescence in HeLa cells. J Virol. 2003;77:6066–6069
  16. Gu W, Putral L, Hengst K, Minto K, Saunders NA, Leggatt G, et al. Inhibition of cell growth in vitro and in vivo with lentiviral vector delivered short hairpin RNA targeting human papilloma virus E6 and E7 oncogenes. Cancer Gene Ther. 2006;13:1023–1032
  17. Aigner A. Delivery systems for the direct application of siRNAs to induce RNA interference (RNAi) in vivo. J Biomed Biotechnol. 2006;2006:1–15
  18. Gilmore IR, Fox SP, Hollins AJ, Sohail M, Akhtar S. The design and exogenous delivery of siRNA for post-transcriptional gene silencing. J Drug Target. 2004;12:315–340
  19. Gilmore IR, Fox SP, Hollins AJ, Sohail M, Akhtar S. Delivery strategies for siRNA-mediated gene silencing. Current Drug Deliv. 2006;3:147–155
  20. Kawakami S, Hashida M. Targeted delivery systems of small interfering RNA by systemic administration. Drug Metabol Pharmacokinet. 2007;22:142–147
  21. Akhtar S, Benter IF. Non viral delivery of synthetic siRNAs in vivo. J Clin Investig. 2007;117:3623–3632
  22. Tripathi PK, Khopade AJ, Dutta T, Ummamaheshwari RB, Jain S, Saraf DK, et al. Dendrimer polycations for the delivery of genes. Indian J Pharm Sci. 2003;65:1–15
  23. Dutta T, Jain NK. Targeting potential and anti HIV activity of mannosylated lamivudine loaded poly(propyleneimine) dendrimer. Biochim Biophys Acta. 2007;1770:681–686
  24. Dutta T, Garg M, Dubey V, Mishra D, Singh K, Pandita D, et al. Toxicological investigations on surface engineered fifth generation poly(propyleneimine) dendrimers in vivo. Nanotoxicology. 2008;2:62–70
  25. Bielinska AV, Latalto JFK, Baker JR. The interaction of plasmid DNA with polyamidoamine dendrimers; mechanism of complex formation and analysis of alternations induced in nuclear sensitivity and transcriptional activity of the complexed DNA. Biochim Biophys Acta. 1997;1353:180–186
  26. Lechardeur D, Sohn KJ, Haardt M, Joshi PB, Monek M, Grahm RW, et al. Metabolic stability of plasmid DNA in the cytosol: A potential barrier to gene transfer. Gene Therapy. 1999;6:482–489
  27. Nishikawa M, Huang L. Non-viral vectors in the new millennium. Delivery barriers in gene transfer. Human Gene Therapy. 2001;12:861–864
  28. Dutta T, Agashe HB, Vijayarajkumar P, Joshi M, Jain NK. Dendrosome based gene delivery. J Exp Nanoscience. 2006;1:235–248
  29. Dutta T, Garg M, Jain NK. Poly (propyleneimine) dendrimer and dendrosome mediated genetic immunization against hepatitis B. Vaccine. 2008;26:3389–3394
  30. Putral L, Bywater M, Gu W, Saunders N, Gabrielli B, Leggatt G, et al. RNA interference against human papillomavirus oncogenes in cervical cancer cells results in increased sensitivity to cisplatin. Mol Pharmacol. 2005;68:1311–1319
  31. Qin L, Pahud DR, Ding Y, Bielinska AV, Kukowska-Lataloo JF, Baker JR, et al. Efficient transfer of genes into murine cardiac graft by starburst PAMAM dendrimers. Hum Gene Ther. 1998;9:553–558
  32. Kang H, DeLong R, Fisher MH, Juliano RL. Tat conjugated PAMAM dendrimers as delivery agents for antisense and siRNA oligonucleotides. Pharm Res. 2005;22:2099–2106
  33. Gregoriadis G, Saffie R, de Souza B. Liposome mediated DNA vaccination. FEBS Lett. 1997;402:107–1110

 The authors thank the National Health and Medical Research Council of Australia for funding support.

PII: S1549-9634(09)00256-1

doi: 10.1016/j.nano.2009.12.001

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 3 , Pages 463-470 , June 2010

Article Tools

* Image Usage & Resolution