Volume 6, Issue 4 , Pages 530-537, August 2010
Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles
Abstract
We developed a method for the determination of three aminothiols—cysteine, glutathione (GSH), and homocysteine—using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). The analytes were first captured using the unmodified 14-nm gold nanoparticles; N-2-mercaptopropionylglycine–modified gold nanoparticles serving as internal standard were sequentially added, and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2 = ∼0.99), with good reproducibility (relative standard deviations: <10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal-standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes.
From the Clinical Editor
This paper reports the development of a surface assisted laser desorption/ionization mass spectrometry method to precisely determine aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys)
Key words: Aminothiols, Drug therapy, Gold nanoparticles, Internal standard, Mass spectrometry
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This study was supported by the National Science Council of Taiwan under contracts NSC 98-2627-M-002-013, NSC 97-2627-M-002-014, and NSC 98-2113-M-002-011-MY3.
PII: S1549-9634(10)00013-4
doi:10.1016/j.nano.2010.01.006
© 2010 Elsevier Inc. All rights reserved.
Volume 6, Issue 4 , Pages 530-537, August 2010
