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Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles

Cheng-Kang Chiang, Yang-Wei Lin, Wen-Tsen Chen, Huan-Tsung ChangCorresponding Author Informationemail address

Received 8 October 2009; received in revised form 15 December 2009; accepted 19 January 2010. published online 05 February 2010.
Accepted Manuscript

Abstract 

We developed a method for the determination of three aminothiols-cysteine (Cys), glutathione (GSH), and homocysteine (HCys)-using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) The analytes were first captured using the unmodified 14-nm Au NPs; N-2-mercaptopropionylglycine (MPG) modified Au NPs served as internal standard was sequentially added and then the sample was analyzed using SALDI-MS. This approach provided good quantitative linearity of the three analytes (R2= ca. 0.99), with good reproducibility (relative standard deviations:<10%), in the analyses of GSH in the lysates of human red blood cells and MCF-7 cancer breast cells in the presence and absence of the anti-inflammatory drug sulfasalazine. The internal standard SALDI-MS approach provides simplicity, accuracy, and precision to the determination of GSH in cells under drug invasion, to open an avenue for SALDI-MS to be used for the precise quantitative determination of a variety of analytes.

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Department of Chemistry, National Taiwan University, Taipei, Taiwan

Corresponding Author InformationCorresponding author. Tel./fax: +011 886 2 33661171.

PII: S1549-9634(10)00013-4

doi:10.1016/j.nano.2010.01.006

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