Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 4 , Pages 530-537 , August 2010

Accurate quantitation of glutathione in cell lysates through surface-assisted laser desorption/ionization mass spectrometry using gold nanoparticles

Received 8 October 2009 ,Accepted 19 January 2010.

References 

  1. Karas M, Hillenkamp F. Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem. 1988;60:2299–2301
  2. Tanaka K, Waki H, Ido Y, Akita S, Yoshida Y, Yoshida T, et al. Protein and polymer analyses up to m/z 100 000 by laser ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom. 1988;2:151–153
  3. Tholey A, Heinzle E. Ionic (liquid) matrices for matrix-assisted laser desorption/ionization mass spectrometry—applications and perspectives. Anal Bioanal Chem. 2006;386:24–37
  4. Sunner J, Dratz E, Chen YC. Graphite surface-assisted laser desorption/ionization time-of-flight mass spectrometry of peptides and proteins from liquid solutions. Anal Chem. 1995;67:4335–4342
  5. Huang YF, Chang HT. Nile red-adsorbed gold nanoparticle matrixes for determining aminothiols through surface-assisted laser desorption/ionization mass spectrometry. Anal Chem. 2006;78:1485–1493
  6. Huang YF, Chang HT. Analysis of adenosine triphosphate and glutathione through gold nanoparticles assisted laser desorption/ionization mass spectrometry. Anal Chem. 2007;79:4852–4859
  7. Chiu TC, Chang LC, Chiang CK, Chang HT. Determining estrogens using surface-assisted laser desorption/ionization mass spectrometry with silver nanoparticles as the matrix. J Am Soc Mass Spectrom. 2008;19:1343–1346
  8. Wen XJ, Dagan S, Wysocki VH. Small-molecule analysis with silicon-nanoparticle-assisted laser desorption/ionization mass spectrometry. Anal Chem. 2007;79:434–444
  9. Lee KH, Chiang CK, Lin ZH, Chang HT. Determining enediol compounds in tea using surface-assisted laser desorption/ionization mass spectrometry with titanium dioxide nanoparticle matrices. Rapid Commun Mass Spectrom. 2007;21:2023–2030
  10. Chen WY, Chen YC. Affinity-based mass spectrometry using magnetic iron oxide particles as the matrix and concentrating probes for SALDI MS analysis of peptides and proteins. Anal Bioanal Chem. 2006;386:699–704
  11. Wang KY, Chuang SA, Lin PC, Huang LS, Chen SH, Ouarda S, et al. Multiplexed immunoassay: quantitation and profiling of serum biomarkers using magnetic nanoprobes and MALDI-TOF MS. Anal Chem. 2008;80:6159–6167
  12. Zhang RJ, Sioma CS, Thompson RA, Xiong L, Regnier FE. Controlling deuterium isotope effects in comparative proteomics. Anal Chem. 2002;74:3662–3669
  13. Gygi SP, Rist B, Gerber SA, Turecek F, Gelb MH, Aebersold R. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat Biotechnol. 1999;17:994–999
  14. Ghezzi P, Romines B, Fratelli M, Eberini I, Gianazza E, Casagrande S, et al. Protein glutathionylation: coupling and uncoupling of glutathione to protein thiol groups in lymphocytes under oxidative stress and HIV infection. Mol Immunol. 2002;38:773–780
  15. Refsum H, Ueland PM, Nygård O, Vollset SE. Homocysteine and cardiovascular disease. Annu Rev Med. 1998;49:31–62
  16. Lai L, Ma L, Chen XJ, Wang GJ, Wang JP, Davey AK. Sensitive LC–ESI–MS method for determination of tiopronin in human plasma. Chromatographia. 2006;64:655–659
  17. Huang MF, Huang CC, Chang HT. Improved separation of double-stranded DNA fragments by capillary electrophoresis using poly(ethylene oxide) solution containing colloids. Electrophoresis. 2003;24:2896–2902
  18. Hettich RL, Buchanan MV, Ho CH. Characterization of photo-induced pyrimidine cyclobutane dimers by laser desorption Fourier transform mass spectrometry. Biomed Environ Mass Spectrom. 1990;19:55–62
  19. Rossi R, Milzani A, Dalle-Donne I, Giustarini D, Lusini L, Colombo R, et al. Blood glutathione disulfide: in vivo factor or in vitro artifact?. Clin Chem. 2002;48:742–753
  20. Narang VS, Pauletti GM, Gout PW, Buckley DJ, Buckley AR. Sulfasalazine-induced reduction of glutathione levels in breast cancer cells: enhancement of growth-inhibitory activity of doxorubicin. Chemotherapy. 2007;53:210–217
  21. Bannai S. Exchange of cystine and glutamate across plasma membrane of human fibroblasts. Biol Chem. 1986;261:2256–2263
  22. Ishii T, Hishinuma I, Bannai S, Sugita Y. Mechanism of growth promotion of mouse lymphoma L1210 cells in vitro by feeder layer or 2-mercaptoethanol. J Cell Physiol. 1981;107:283–293
  23. Collins JA, Xirouchaki C, Palmer RE, Heath JK, Jones CH. Clusters for biology: immobilization of proteins by size-selected metal clusters. Appl Surf Sci. 2004;226:197–208
  24. Lee KH, Chen SJ, Jeng JY, Cheng YC, Shiea JT, Chang HT. Fluorescence and interactions with thiol compounds of Nile Red-adsorbed gold nanoparticles. J Colloids Interf Sci. 2007;307:340–348

 This study was supported by the National Science Council of Taiwan under contracts NSC 98-2627-M-002-013, NSC 97-2627-M-002-014, and NSC 98-2113-M-002-011-MY3.

PII: S1549-9634(10)00013-4

doi: 10.1016/j.nano.2010.01.006

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 4 , Pages 530-537 , August 2010