Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 4 , Pages 583-589, August 2010

Hepatoma-targeted gene delivery using a tumor cell–specific gene regulation system combined with a human liver cell–specific bionanocapsule

  • Jeong-Hun Kang, PhD

      Affiliations

    • Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan
    • These authors contributed equally to this work.
  • ,
  • Jun Oishi, PhD

      Affiliations

    • Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan
    • Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan
    • These authors contributed equally to this work.
  • ,
  • Jong-Hwan Kim, PhD

      Affiliations

    • Faculty of Education, Nagasaki University, Nagasaki, Japan
  • ,
  • Moeko Ijuin, MD

      Affiliations

    • Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan
    • Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan
  • ,
  • Riki Toita, MD

      Affiliations

    • Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan
    • Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan
  • ,
  • Byungdug Jun, PhD

      Affiliations

    • Faculty of Education, Nagasaki University, Nagasaki, Japan
  • ,
  • Daisuke Asai, PhD

      Affiliations

    • Department of Microbiology, St. Marianna University School of Medicine, Kawasaki, Japan
  • ,
  • Takeshi Mori, PhD

      Affiliations

    • Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan
    • Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan
  • ,
  • Takuro Niidome, PhD

      Affiliations

    • Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan
    • Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan
    • Center for Future Chemistry, Kyushu University, Fukuoka, Japan
  • ,
  • Katsuyuki Tanizawa, PhD

      Affiliations

    • Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan
  • ,
  • Shun'ichi Kuroda, PhD

      Affiliations

    • Institute of Scientific and Industrial Research, Osaka University, Osaka, Japan
  • ,
  • Yoshiki Katayama, PhD

      Affiliations

    • Department of Applied Chemistry, Faculty of Engineering, Kyushu University, Fukuoka, Japan
    • Graduate School of Systems Life Sciences, Kyushu University, Fukuoka, Japan
    • Center for Future Chemistry, Kyushu University, Fukuoka, Japan
    • Corresponding Author InformationCorresponding author: Department of Applied Chemistry, Faculty of Engineering, Kyushu University, 744 Motooka, Nishi-Ku, Fukuoka 819-0395, Japan.

Received 4 July 2009; accepted 15 January 2010. published online 05 February 2010.

Abstract 

Hepatoma (hepatocellular carcinoma) is the most common type of malignant tumor originating in the liver and has a relatively low 5-year survival rate. The development of hepatoma-targeted therapy is needed to increase treatment efficiency and to reduce the incidence of undesirable side effects. In this study we developed a novel hepatoma-targeted gene delivery system. The gene delivery system was prepared by combining a human liver cell–specific bionanocapsule (BNC) and a tumor cell–specific gene regulation polymer, which responds to hyperactivated protein kinase Cα in hepatoma cells. The complex of the polymer-DNA with BNCs was delivered into cells and tissues. The developed system showed increased transfection efficiency and resulted in cell-specific gene expression in hepatoma cells and tissues (HuH-7), but no gene expression in normal human hepatocytes or human epidermoid tumor cells (A431). The combination of a tumor cell-specific gene regulation system responding to protein kinase Cα and BNCs showed excellent potential for the selective treatment of hepatomas. The system could be a useful method with applications in hepatoma-specific gene therapy and molecular imaging.

From the Clinical Editor

Hepatocellular carcinoma is the most common type of malignant tumor in the liver with a low 5-year survival rate. In this study, a novel hepatoma-targeted gene delivery system was prepared by combining a human liver cell-specific bionanocapsule and a tumor cell-specific gene regulation polymer, which responds to hyperactivated protein kinase C (PKC)a in hepatoma cells. The system could be a useful in hepatoma-specific gene therapy and molecular imaging.

Key words: Protein kinase, Intracellular signal, Hepatocellular carcinoma, Gene delivery

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 This work was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan.

PII: S1549-9634(10)00014-6

doi:10.1016/j.nano.2010.01.007

Refers to erratum:

  • Erratum to “Hepatoma-targeted gene delivery using a tumor cell–specific gene regulation system combined with a human liver cell–specific bionanocapsule” [Nanomed Nanotechnol Biol Med. 2010;6:583-9] , 18 July 2011

    Jeong-Hun Kang, Jun Oishi, Jong-Hwan Kim, Moeko Ijuin, Riki Toita, Byungdug Jun, Daisuke Asai, Takeshi Mori, Takuro Niidome, Katsuyuki Tanizawa, Shun'ichi Kuroda, Yoshiki Katayama
    Nanomedicine: Nanotechnology, Biology and Medicine August 2011 (Vol. 7, Issue 4, Page 514)

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 4 , Pages 583-589, August 2010