In vitro evaluation of novel polymer-coated magnetic nanoparticles for controlled drug delivery

Rahimi, Maham; Wadajkar, Aniket; Subramanian, Khaushik; Yousef, Monet; Cui, Weina; Hsieh, Jer-Tsong; Nguyen, Kytai Truong

doi:10.1016/j.nano.2010.01.012

« Previous Next »Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 5 , Pages 672-680, October 2010

In vitro evaluation of novel polymer-coated magnetic nanoparticles for controlled drug delivery

Maham Rahimi, PhD
- Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA and University of Texas at Arlington, Arlington, Texas, USA
- Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA
Aniket Wadajkar, MS
- Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA and University of Texas at Arlington, Arlington, Texas, USA
- Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA
Khaushik Subramanian, MS
- Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA and University of Texas at Arlington, Arlington, Texas, USA
- Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA
Monet Yousef, BS
- Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA and University of Texas at Arlington, Arlington, Texas, USA
- Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA
Weina Cui, PhD
- Department of Radiology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
Jer-Tsong Hsieh, PhD
- Department of Urology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA
- Graduate Institute of Cancer Biology, China Medical University, Taichung, Taiwan
Kytai Truong Nguyen, PhD
- Joint Biomedical Engineering Program, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas, USA and University of Texas at Arlington, Arlington, Texas, USA
- Department of Bioengineering, University of Texas at Arlington, Arlington, Texas, USA
- Corresponding author: 501 West First Street, ELB 220, Arlington, Texas 76019, USA.

Received 15 September 2009; accepted 25 January 2010. published online 22 February 2010.

Abstract

Previously uncharacterized poly(N-isopropylacrylamide-acrylamide-allylamine)-coated magnetic nanoparticles (MNPs) were synthesized using silane-coated MNPs as a template for radical polymerization of N-isopropylacrylamide, acrylamide, and allylamine. Properties of these nanoparticles such as size, biocompatibility, drug loading efficiency, and drug release kinetics were evaluated in vitro for targeted and controlled drug delivery. Spherical core-shell nanoparticles with a diameter of 100 nm showed significantly lower systemic toxicity than did bare MNPs, as well as doxorubicin encapsulation efficiency of 72%, and significantly higher doxorubicin release at 41°C compared with 37°C, demonstrating their temperature sensitivity. Released drugs were also active in destroying prostate cancer cells (JHU31). Furthermore, the nanoparticle uptake by JHU31 cells was dependent on dose and incubation time, reaching saturation at 500 μg/mL and 4 hours, respectively. In addition, magnetic resonance imaging capabilities of the particles were observed using agarose platforms containing cells incubated with nanoparticles. Future work includes investigation of targeting capability and effectiveness of these nanoparticles in vivo using animal models.

From the Clinical Editor

In this paper, previously uncharacterized magnetic nanoparticles were synthesized using silane-coated MNPs as a template for radical polymerization of N-isopropylacrylamide, acrylamide, and allylamine. Various properties of these nanoparticles were evaluated in vitro for targeted drug delivery.

Key words: Magnetic nanoparticles, Temperature-responsive polymers, Prostate cancer, Doxorubicin

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Financial support was provided by U.S. Department of Defense Idea Development Award W81XWH-09-1-0313 (K.N.), Flight Attendants Medical Research Institute (J-T.H), and the Small Animal Imaging Resource Program (U24 CA126608) and the Biological Threat Reduction Program (P41-RR02584) to W.C.

PII: S1549-9634(10)00019-5

doi:10.1016/j.nano.2010.01.012

« Previous Next »Nanomedicine: Nanotechnology, Biology and Medicine
Volume 6, Issue 5 , Pages 672-680, October 2010

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