Nanomedicine: Nanotechnology, Biology and Medicine
Volume 7, Issue 4 , Pages 376-384, August 2011

Multistability in platelets and their response to gold nanoparticles

  • Suryyani Deb, MSc

      Affiliations

    • Department of Biochemistry, University of Calcutta, Kolkata, India
    • ,
  • Hirak K. Patra, MSc

      Affiliations

    • Department of Biochemistry, University of Calcutta, Kolkata, India
    • ,
  • Prabir Lahiri, PhD

      Affiliations

    • Department of Biochemistry, University of Calcutta, Kolkata, India
    • ,
  • Anjan Kr. Dasgupta, PhD

      Affiliations

    • Department of Biochemistry, University of Calcutta, Kolkata, India
    • Corresponding Author InformationCorresponding author: Department of Biochemistry, University of Calcutta, 35 Ballygunge Circular Road, Kolkata 700019, India
    • ,
  • Kuntal Chakrabarti, PhD

      Affiliations

    • Department of Materials Science, S.N.Bose National Centre for Basic Sciences, Salt Lake, Kolkata, India
    • ,
  • Utpal Chaudhuri, MD

      Affiliations

    • Institute of Hematology and Transfusion Medicine, Calcutta Medical College, Kolkata, India

Received 25 June 2010; accepted 16 January 2011. published online 09 February 2011.

Abstract 

The nanoparticle (NP) response of platelets is shown to be critically dependent on extent of preactivation of platelets by an agonist like ADP. A transition from de-aggregatory to aggregatory state is triggered in the presence of gold NPs (AuNP) only in such critical conditions. Adhered and suspended platelets respond differentially to NPs. Preactivation in the adhered state induced by shear force explains such observation. The NP effect is associated with enhanced release reaction, tyrosine phosphorylation and CD62P expression level. Unlike cancer cells, whose response is maximal when NP size is optimal (within the range 50 - 70 nm), the platelet response monotonically increases with reduction of the AuNP size. The uptake study, using quenching of quinacrine hydrochloride fluorescence by AuNP, indicates that accumulation 18 nm AuNP is several-fold higher than the 68 nm AuNP. It is further shown that AuNP response can provide a simple measure for thrombotic risk associated with nano-drugs.

From the Clinical Editor

Platelet aggregation can be triggered in the presence of gold nanoparticles (AuNP). Platelet response monotonically increases with reduction of the AuNP size. AuNP response can provide a simple measure for thrombotic risk associated with nano-drugs.

Graphical Abstract 

The logical course of events that follow when platelet is exposed to AuNP, the nanoparticle size effect, the critical agonist effect the differential response for adhered and supended platelets and the mechanism of the nanoparticle induced pro-thrombotic effect (involving release reaction) are illustrated.

Key words: Gold nanoparticle, Multi-stability, Granule release, Adhesion, Activation, Nanosafety measure, Thrombotic risk

 

 Funding Source: DBT, Govt. of India. (Grant No. BT/PR9953/MNT/28/73/2007).

PII: S1549-9634(11)00011-6

doi:10.1016/j.nano.2011.01.007

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 7, Issue 4 , Pages 376-384, August 2011

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