Nanomedicine: Nanotechnology, Biology and Medicine
Volume 8, Issue 1 , Pages 93-102 , January 2012

The effects of cancer progression on the viscoelasticity of ovarian cell cytoskeleton structures

  • Alperen N. Ketene, BSc

      Affiliations

    • Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia, USA
    • VT MEMS Lab, The Bradley Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg, Virginia, USA
    • ,
  • Eva M. Schmelz, PhD

      Affiliations

    • Department of Human Nutrition, Foods and Exercise, Virginia Tech, Blacksburg, Virginia, USA
    • ,
  • Paul C. Roberts, PhD

      Affiliations

    • Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia, USA
    • ,
  • Masoud Agah, PhD

      Affiliations

    • VT MEMS Lab, The Bradley Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg, Virginia, USA
    • Corresponding Author InformationCorresponding author: The Bradley Department of Electrical and Computer Engineering, Virginia Tech, Blacksburg, VA, 24061.

Received 4 February 2011 ,Accepted 19 May 2011.

  • Image Result

    Optical microscope view screenshot taken during actual AFM experimentation. All indentations were performed on randomly selected cells that had visually “spread” morphologies.

    Optical microscope view screenshot taken during actual AFM experimentation. All indentations were performed on randomly selected cells that had visually “spread” morphologies.

  • Image Result

    (A) Explanatory graph showing the full AFM stress relaxation procedure. (B) Force curves obtained via force curve technique on MOSE cells (C) Stress relaxation response curves on MOSE cells. It was vi

    (A) Explanatory graph showing the full AFM stress relaxation procedure. (B) Force curves obtained via force curve technique on MOSE cells (C) Stress relaxation response curves on MOSE cells. It was visually possible to distinguish between the cell responses because of the precise sensitivity of the AFM.

  • Image Result
    MOSE cell line histograms depicting the change in distribution of recorded elastic modulus from one stage of cancer to another.

    MOSE cell line histograms depicting the change in distribution of recorded elastic modulus from one stage of cancer to another.

  • Image Result
    MOSE cell line histograms depicting the change in distribution of recorded viscosity rates from one stage of cancer to another.

    MOSE cell line histograms depicting the change in distribution of recorded viscosity rates from one stage of cancer to another.

  • Image Result
    Depiction of change in both elastic modulus and apparent viscosity parameters for the MOSE cell line during cancer progression.

    Depiction of change in both elastic modulus and apparent viscosity parameters for the MOSE cell line during cancer progression.

  • Image Result
    Confocal images of MOSE early- and late-stage cells. A 20x objective was used in air to obtain these results. The images show the early-stage cells as having a much denser concentration of actin stres

    Confocal images of MOSE early- and late-stage cells. A 20x objective was used in air to obtain these results. The images show the early-stage cells as having a much denser concentration of actin stress fibers when compared with their diseased counterparts.

 Conflict of interest: No conflict of interest was reported by the authors of this paper.

 E.M.S. and P.C.R. are supported in part by NIH/NCI CA118846. This study was made possible by the support of the National Science Foundation (NSF) Award No. ECCS-IDR 0925945 as well as the Institute for Critical Technology and Applied Science (ICTAS).

PII: S1549-9634(11)00184-5

doi: 10.1016/j.nano.2011.05.012

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 8, Issue 1 , Pages 93-102 , January 2012

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