Nanomedicine: Nanotechnology, Biology and Medicine
Volume 8, Issue 2 , Pages 167-175 , February 2012

Linear PEI nanoparticles: efficient pDNA/siRNA carriers in vitro and in vivo

  • Ritu Goyal, MPhil

      Affiliations

    • CSIR-Institute of Genomics and Integrative Biology, Delhi University, Delhi, India
  • ,
  • Sushil K. Tripathi, MSc

      Affiliations

    • CSIR-Institute of Genomics and Integrative Biology, Delhi University, Delhi, India
  • ,
  • Shilpa Tyagi, MSc

      Affiliations

    • CSIR-Indian Institute of Toxicology Research, Lucknow, India
  • ,
  • Anurag Sharma, MSc

      Affiliations

    • CSIR-Indian Institute of Toxicology Research, Lucknow, India
  • ,
  • K. Ravi Ram, PhD

      Affiliations

    • CSIR-Indian Institute of Toxicology Research, Lucknow, India
  • ,
  • Debapratim K. Chowdhuri, PhD

      Affiliations

    • CSIR-Indian Institute of Toxicology Research, Lucknow, India
  • ,
  • Yogeshwar Shukla, PhD

      Affiliations

    • CSIR-Indian Institute of Toxicology Research, Lucknow, India
  • ,
  • P. Kumar, PhD

      Affiliations

    • CSIR-Institute of Genomics and Integrative Biology, Delhi University, Delhi, India
  • ,
  • Kailash C. Gupta, PhD

      Affiliations

    • CSIR-Institute of Genomics and Integrative Biology, Delhi University, Delhi, India
    • CSIR-Indian Institute of Toxicology Research, Lucknow, India
    • Corresponding Author InformationCorresponding author: Indian Institute of Toxicology Research, Mahatma Gandhi Marg, Lucknow-226001, India.

Received 16 October 2010 ,Accepted 2 June 2011.

References 

  1. Neu M, Fischer D, Kissel T. Recent advances in rational gene transfer vector design based on poly(ethylenimine) and its derivatives. J Gene Med. 2005;7:992–1009
  2. Kim TI, Baek JU, Zhe BC, Park JS. Arginine-conjugated polypropylenimine dendrimer as a non-toxic and efficient gene delivery carrier. Biomaterials. 2007;28:2061–2067
  3. Pathak A, Aggarwal A, Kurupati RK, Patnaik S, Swami A, Singh Y, et al. Engineered polyallylamine nanoparticles for efficient in vitro transfection. Pharm Res. 2007;24:1427–1440
  4. Hosseinkhani H, Azzam T, Tabata Y, Domb AJ. Dextran-spermine polycation: an efficient non-viral vector for in vitro and in vivo gene transfection. Gene Ther. 2004;11:194–203
  5. Erbacher P, Zou S, Bettinger T, Steffan AM, Remy JS. Chitosan based vector/DNA complexes for gene delivery: Biophysical characteristics and transfection ability. Pharm Res. 1998;15:1332–1339
  6. Lungwitz U, Breunig M, Blunk T, Gopferich A. Polyethylenimine based non-viral gene delivery systems. Eur J Pharm Biopharm. 2005;60:247–266
  7. Tang MX, Szoka FC. The influence of polymer structure on the interactions of cationic polymers with DNA and morphology of the resulting complexes. Gene Ther. 1997;4:823–832
  8. Kircheis R, Wightman L, Wagner E. Design and gene delivery activity of modified polyethylenimines. Adv Drug Delivery Rev. 2001;53:341–358
  9. Sonawane ND, Szoka FC, Verkman AS. Chloride accumulation and swelling in endosomes enhances DNA transfer by polyamine-DNA polyplexes. J Biol Chem. 2003;278:44826–44831
  10. Kunath K, von Harpe A, Fischer D, Petersen H, Bickel U, Voight K, et al. Low-molecular-weight polyethylenimine as a non-viral vector for DNA delivery: Comparison of physicochemical properties, transfection efficiency and in vivo distribution with high-molecular-weight polyethylenimine. J Control Release. 2003;89:113–125
  11. Zhang C, Yadava P, Hughes J. Polyethylenimine strategies for plasmid delivery to brain-derived cells. Methods. 2004;33:144–150
  12. Durocher Y, Perret S, Kamen A. High-level and high-throughput recombinant protein production by transient transfection of suspension- growing human 293-EBNA1 cells. Nucl Acids Res. 2002;30:E9
  13. Gao X, Liu D. Selective chemical modification on polyethylenimine and its effects on transfection efficiency and cytotoxicity. Mol Ther. 2005;11:S427–S428
  14. Seib FP, Jones AT, Duncan R. Comparison of the endocytic properties of linear and branched PEIs, and cationic PAMAM dendrimers in B16f10 melanoma cells. J Control Release. 2007;117:291–300
  15. Zhong Z, Feijen J, Lok MC, Hennink WE, Christensen LV, Yockman JW, et al. Low molecular weight linear polyethylenimine-b-poly(ethylene glycol)-b-polyethylenimine triblock copolymers: synthesis, characterization, and in vitro gene transfer properties. Biomacromolecules. 2005;6:3440–3448
  16. Banerjee P, Weissleder R, Bogdanov A. Linear polyethyleneimine grafted to a hyperbranched poly(ethylene glycol)-like core: a copolymer for gene delivery. Bioconjug Chem. 2006;17:125–131
  17. Hsiue GH, Chiang HZ, Wang CH, Juang TM. Nonviral gene carriers based on diblock copolymers of poly(2-ethyl-2-oxazoline) and linear polyethylenimine. Bioconjug Chem. 2006;17:781–786
  18. Pons B, Mouhoubi L, Adib A, Godzina P, Behr JP, Zuber G. ω-Hydrazino linear polyethylenimine: a monoconjugation building block for nucleic acid delivery. ChemBioChem. 2006;7:303–309
  19. Liu X, Jennifer WY, Lynn DM. Addition of “charge-shifting” side chains to linear poly(ethyleneimine) enhances cell transfection efficiency. Biomacromolecules. 2008;9:2063–2071
  20. Bonnet ME, Erbacher P, Bolcato-Bellemin AL. Systemic delivery of DNA or siRNA mediated by linear polyethylenimine (L-PEI) does not induce an inflammatory response. Pharm Res. 2008;25:2972–2982
  21. Pun SH, Bellocq NC, Liu A, Jensen G, Machemer T, Quijano E, et al. Cyclodextrin-modified polyethylenimine polymers for gene delivery. Bioconjug Chem. 2004;15:831–840
  22. Shuai X, Merdan T, Unger F, Kissel T. Supramolecular gene delivery vectors showing enhanced transgene expression and good biocompatibility. Bioconjug Chem. 2005;16:322–329
  23. Yamashita A, Choi HS, Ooya T, Yui N, Akita H, Kogure K, et al. Improved cell viability of linear polyethylenimine through γ-cyclodextrin inclusion for effective gene delivery. Chem Bio Chem. 2006;7:297–302
  24. Swami A, Kurupati RK, Pathak A, Singh Y, Kumar P, Gupta KC. A unique and highly efficient non-viral DNA/siRNA delivery system based on PEI-bisepoxide nanoparticles. Biochem Biophys Res Commun. 2007;362:835–841
  25. Matsumoto I, Kitagaki H, Akai Y, Ito Y, Seno N. Derivatization of epoxy-activated agarose with various carbohydrates for the preparation of stable and high-capacity affinity adsorbants: their use for affinity chromatography of carbohydrate-binding proteins. Anal Biochem. 1981;116:103–110
  26. Tseng WC, Tang CH, Fang TY. The role of dextran conjugation in transfection mediated by dextran-grafted polyethylenimine. J Gene Med. 2004;6:895–905
  27. Grasemann H, Ratjen F. Emerging therapies for cystic fibrosis lung disease. Expert Opin Emerg Drugs. 2010;15:653–659
  28. Fiandaca MS, Bankiewicz KS. Gene therapy for Parkinson's disease: from non-human primates to humans. Curr Opin Mol Ther. 2010;12:519–529
  29. Hashemi M, Parhiz BH, Hatefi A, Ramezani M. Modified polyethyleneimine with histidine–lysine short peptides as gene carrier. Cancer Gene Therapy. 2011;18:12–19
  30. Liu J, Jiang X, Xu L, Wang X, Hennink WE, Zhuo R. Novel reduction-responsive cross-linked polyethylenimine derivatives by click chemistry for nonviral gene delivery. Bioconjug Chem. 2010;21:1827–1835
  31. Kunath K, von Harpe A, Fischer D, Kissel T. Galactose-PEI-DNA complexes for targeted gene delivery: degree of substitution affects complex size and transfection efficiency. J Control Release. 2003;88:159–172
  32. Godbey WT, Wu KK, Mikos AG. Poly(ethylenimine) and its role in gene delivery. J Control Release. 1999;60:149–160
  33. Kichler A, Leborgne C, Coeytaux E, Danos O. Polyethylenimine-mediated gene delivery: a mechanistic study. J Gene Med. 2001;3:135–144
  34. Swami A, Goyal R, Tripathi SK, Singh N, Katiyar N, Mishra AK, et al. Effect of homobifunctional crosslinkers on nucleic acids delivery ability of PEI nanoparticles. Int J Pharm. 2009;374:125–138
  35. Bertschinger M, Backliwal G, Schertenleib A, Jordan M, Hacker DL, Wurm FM. Disassembly of polyethylenimine-DNA particles in vitro: implications for polyethylenimine-mediated DNA delivery. J Control Release. 2006;116:96–104
  36. Ganesh KN, Sastry M. DNA-amine interactions: from monolayers to nanoparticles. J Ind Inst Sci. 2002;82:105–112
  37. Vijayanathan V, Thomas TJ. DNA nanoparticles and development of DNA delivery vehicles for gene therapy. Biochemistry. 2002;41:14085–14094
  38. Boussif O, Lezoualch F, Zanta MA, Mergny MD, Scherman D, Demeneix B, et al. A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo polyethylenimine. Proc Nat Acad Sci USA. 1995;92:7297–7301
  39. Jeong JH, Song SH, Lim DW, Lee H, Park TG. DNA transfection using linear poly(ethylenimine) prepared by controlled acid hydrolysis of poly(2-ethyl-2-oxazoline). J Control Release. 2001;73:391–399
  40. Brissault B, Kichler A, Guis C, Leborgne C, Danos O, Cheradame H. Synthesis of linear polyethylenimine derivatives for DNA transfection. Bioconjug Chem. 2003;14:581–587
  41. Singh MP, Reddy MM, Saxena MN, Chowdhuri DK. Induction of hsp70, hsp60, hsp83 and hsp26 and oxidative stress markers in benzene, toluene and xylene exposed Drosophila Melanogaster: role of ROS generation. Toxicol Appl Pharmacol. 2009;235:226–243
  42. Brissault B, Leborgne C, Guis C, Danos O, Cheradame H, Kichler A. Linear topology confers in vivo gene transfer activity to polyethylenimines. Bioconjug Chem. 2006;17:759–765
  43. Ahamed M, Posgai R, Gorey TJ, Nielsen M, Hussain SM, Rowe JJ. Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila Melanogaster. Toxicol Appl Pharmacol. 2010;242:263–269
  44. Breunig M, Lungwitz U, Liebl R, Klar J, Obermayer B, Blunk T, et al. Mechanistic insights into linear polyethylenimine-mediated gene transfer. Biochim Biophys Acta. 2007;1770:196–205
  45. Kurosaki T, Kitahara T, Kawakami S, Higuchi Y, Yamaguchi A. Nakagawa H, et al. γ-Polyglutamic acid-coated vectors for effective and safe gene therapy. J Control Release. 2009;142:404–410
  46. Wolff JA, Malone RW, Williams P, Chong W, Acsadi G, Jani A, et al. Direct gene transfer into mouse muscle in vivo. Science. 1990;247:1465–1468
  47. Merdan T, Kopecek J, Kissel T. Prospects for cationic polymers in gene and oligonucleotide therapy against cancer. Adv Drug Delivery Rev. 2002;54:715–758

 No conflict of interest was reported by the authors of this article.

 Financial support from the CSIR (NWP-035) and UGC-SRF (10-2(05)2006(i)-E.U.II) to RG is gratefully acknowledged.

PII: S1549-9634(11)00254-1

doi: 10.1016/j.nano.2011.06.001

Nanomedicine: Nanotechnology, Biology and Medicine
Volume 8, Issue 2 , Pages 167-175 , February 2012