Electrostatic assembly of a DNA superparamagnetic nano-tool for simultaneous intracellular delivery and in situ monitoring
Abstract
A superparamagnetic γFe2O3 nanocarrier was developed, characterized by spectroscopic methods and evaluated for the delivery of a decoy oligonucleotide (dODN) in human colon carcinoma SW 480 cells. This nanoparticle-dODN bioconjugate (γFe2O3@dODN) was designed to target the signal transducer and activator of transcription 3, STAT3, a key regulator of cell survival and proliferation. We exploited a simple precipitation-redispersion mechanism for the direct and one-step complexation of a labeled decoy oligonucleotide with iron oxide nanoparticles (NPs). The cell internalization of the decoy γFe2O3@dODN nanoparticles is demonstrated and suggests the potential for DNA delivery in biological applications. Despite the increasing use of NPs in biology and medicine, convenient methods to quantify them within cells are still lacking. In this work, taking advantage of the nonlinear magnetic behavior of our superparamagnetic NPs, we have developed a new method to quantify in situ their internalization by cells.
Graphical Abstract
A superparamagnetic gFe2O3 nanocarrier was developed combining a delivery system of an oligonucleotide and a magnetic nanoprobe for in-situ monitoring. Such dual nanosystem was designed for targeting the signal transducer and activator of transcription 3, STAT3, a key regulator of cell survival and proliferation.
Key words: DNA, Decoy ODN, Delivery nanosystem, Nanoprobes, Superparamagnetic nanoparticle
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No conflict of interest was reported by the authors of this article.
PII: S1549-9634(12)00004-4
doi:10.1016/j.nano.2011.12.010
© 2012 Published by Elsevier Inc.
