Research ArticleEnhancement of cell permeabilization apoptosis-inducing activity of selenium nanoparticles by ATP surface decoration
Graphical abstract
ATP surface decoration significantly enhances the cellular uptake and anticancer activity of SeNPs. Induction of apoptosis was the major mode of cancer cell death induced by SeNPs@ATP. Our results demonstrate that it is a novel strategy to use ATP as a surface decorator of SeNPs to achieve anticancer synergism. SeNPs@ATP may be a candidate for further evaluation as a therapeutic agent for human cancers.
Section snippets
Preparation of SeNPs@ATP
Aqueous ATP (Sigma-Aldrich, St. Louis, Missouri) solution (50 mM, 4 ml) was mixed with 2 mL 100 mM ascorbic acid (Sigma-Aldrich) and 13.5 mL Milli-Q water under magnetic stirring. Aqueous sodium selenite (Sigma-Aldrich) solution (40 mM, 0.5 ml) was slowly added into the mixtures. After reaction for 5 minutes under sonication, the solutions were dialyzed against Milli-Q water at 4°C until no Se was detected in the outer solutions as determined by ICP-AES (Inductively Coupled Plasma-Atomic
Preparation and characterization of SeNPs@ATP
This article summarizes the present study, in which we demonstrated a simple method to synthesize functionalized SeNPs through conjugation of ATP to the surface of Se nanoparticles. Figure 1 shows the representative TEM images of the as-prepared SeNPs with the absence (A) and presence (B) of 10 mM ATP, which clearly revealed that SeNPs@ATP presented monodisperse and homogeneous spherical structure with an average diameter of about 40 nm, whereas SeNPs alone in aqueous solutions were badly
Discussion
The development of cancer-nanotechnology has opened the new horizons for cancer imaging, diagnosis, and targeted therapy.1, 2, 29 Numerous nanomaterials have been applied in cancer imaging, diagnosis, and therapy due to their unique optical, magnetic, radiological, and structural properties.1, 2, 4, 29, 30, 31 For instance, great achievements have been made by using nanoscale liposomes to deliver Zoledronic acid.32, 33, 34, 35 Carbon nanotubes have also been proved to be an excellent drug
References (50)
- et al.
Combination therapy: Opportunities and challenges for polymer-drug conjugates as anticancer nanomedicines. Adv Drug Deliv Rev
(2009) - et al.
Antioxidant idebenone-loaded nanoparticles based on chitosan and N-carboxymethylchitosan
Nanomedicine
(2010) - et al.
Targeted epidermal growth factor receptor nanoparticle bioconjugates for breast cancer therapy
Biomaterials
(2009) - et al.
Cell selective response to gold nanoparticles
Nanomedicine
(2007) The importance of selenium to human health
Lancet
(2000)- et al.
Chemical forms of selenium for cancer prevention
J Trace Elem Med Biol
(2005) - et al.
Free radical scavenging efficiency of Nano-Se in vitro
Free Radic Biol Med
(2003) - et al.
Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: Comparison with selenomethionine in mice
Free Radic Biol Med
(2007) - et al.
Selenium nanoparticles fabricated in Undaria pinnatifida polysaccharide solutions induce mitochondria-mediated apoptosis in A375 human melanoma cells
Colloids Surf B Biointerfaces
(2008) - et al.
P2 receptors and cancer
Trends Pharmacol Sci
(2006)
ATP-induced apoptosis involves a Ca2+-independent phospholipase A(2) and 5-lipoxygenase in macrophages
Prostaglandins Other Lipid Mediat
The involvement of cellular ATP in receptor-mediated internalization of epidermal growth-factor and hormone-induced internalization of beta-adrenergic receptors
J Biol Chem
Selenocystine induces reactive oxygen species-mediated apoptosis in human cancer cells
Biomed Pharmacother
Mitochondria-mediated apoptosis in human breast carcinoma MCF-7 cells induced by a novel selenadiazole derivative
Biomed Pharmacother
Preparation of (3)H-ATP-incorporated silica nanoparticles and its diffusive release
J Non-Cryst Solids
In vitro degradation of insulin-loaded poly (n-butylcyanoacrylate) nanoparticles
Biomaterials
Self-assembly nanoparticles for the delivery of bisphosphonates into tumors
Int J Pharmaceut
Nanotechnologies to use zoledronic acid as a potent antitumoral agent
J Drug Deliv Sci Technol
Nanotechnologies to use bisphosphonates as potent anticancer agents: the effects of zoledronic acid encapsulated into liposomes
Nanomedicine
Sialic acid surface decoration enhances cellular uptake and apoptosis-inducing activity of selenium nanoparticles
Colloids Surf B Biointerfaces
Functionalization of liposomes with ApoE-derived peptides at different density affects cellular uptake and drug transport across a blood-brain barrier model
Nanomedicine
A diverse family of proteins containing tumor necrosis factor receptor-associated factor domains
J Biol Chem
Mitochondria superoxide dismutase mimetic inhibits peroxide-induced oxidative damage and apoptosis: Role of mitochondrial superoxide
Free Rad Biol Med
Selenocystine induces caspase-independent apoptosis in MCF-7 human breast carcinoma cells with involvement of p53 phosphorylation and reactive oxygen species generation
Int J Biochem Cell Biol
Reactive oxygen species, antioxidants, and the mammalian thioredoxin system
Free Rad Biol Med
Cited by (148)
Adenosine Triphosphatase-Stabilized ZIF-67 as Peroxidase Mimic for Rapid Detection of Glutathione
2024, Inorganic Chemistry CommunicationsSignal profile of synthesized nano CuAlO<inf>2</inf> on human skin epidermoid carcinoma epithelial cells
2022, Materials Today CommunicationsPreparation, characterization, and antitumor activity of Chaenomeles speciosa polysaccharide-based selenium nanoparticles
2022, Arabian Journal of ChemistryBiomaterials as therapeutic drug carriers for inflammatory bowel disease treatment
2022, Journal of Controlled ReleaseCitation Excerpt :Recently, selenium nanoparticles (SeNPs) have appeared to be more effective in increasing selenoprotein levels than other forms of selenium. Numerous surface modification methods, such as capping via ATP or vitamin C, were introduced as effective strategies to enhance the stability, cellular uptake, and size control of SeNPs [156]. To further potentiate SeNPs as IBD attenuating materials, Zhu et al. decorated the surface of SeNPs with ulca lactuca polysaccharide, a material known to possess immunomodulatory, antioxidant, and surfactant properties [157,158].
No conflict of interest was reported by the authors of this paper.
This work was supported by Natural Science Foundation of China and Guangdong Province, Program for New Century Excellent Talents in University, Key Project of Science and Technology Department of Guangdong Province, the Fundamental Research Funds for the Central Universities and Grant of State Key Laboratory of Oncology in South China.