Original ArticleNear infra-red laser mediated photothermal and antitumor efficacy of doxorubicin conjugated gold nanorods with reduced cardiotoxicity in swiss albino mice
Graphical Abstract
NIR laser mediated photothermal and antitumor efficacy of doxorubicin conjugated gold nanorods against Dalton lymphoma ascites tumor with reduced cardiotoxicity in Swiss albino mice.
Section snippets
Synthesis and characterization of DOX@PSS-GNR
Monodisperse GNR was synthesized using the silver ion-assisted seed mediated growth method and were surface modified with anionic PSS as described in our previous report.20 The detailed methodology for synthesis and characterization of DOX@PSS-GNR is provided in supporting information. For all dilutions, DOX@PSS-GNR was dispersed in phosphate buffered saline (pH = 7.4).
Animals and tumor models
Six to eight week old male Swiss albino mice (20-25 g BW) were purchased from IISC Bangalore, India and were maintained in the
Results
In the present study, we demonstrate the in vivo antitumor effect of DOX@PSS-GNR along with its photothermal efficiency against DLA tumor in Swiss albino mice. Synthesis and characterization of DOX@PSS-GNR have been briefly explained in the supporting information. Preliminary results of the study indicate the highly biocompatible nature of DOX@PSS-GNR with excellent in vitro stability in serum sample as evidenced by hemolytic assay (Figure SI). The loading efficiency of DOX molecule on GNR-PSS
Discussion
Recently we reported a multifunctional DOX delivery system by conjugating doxorubicin with GNR via poly (sodium 4-styrensulfonate) (PSS) and found it to be highly biocompatible with excellent anticancer potential against human breast cancer cells, in vitro.20 In continuation of the study, herein we investigate the in vivo antitumor and photothermal efficacy of DOX@PSS-GNR along with its potential impact on cardiotoxicity in Swiss albino mice. The entire study has been carried out employing a
Acknowledgment
The authors are grateful to Ms. H. Kalpana, Mr. S. Venkatesh and Mr. V. Raju (Department of Basic Medical Science and Physics, Bharathidasan University, Tiruchirappalli) for technical support.
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Source of Support: This work was supported by the Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, New Delhi, India under the grant no. BT/PR12864/NNT/28/442/2009.
Conflict of interest statement: None of the authors declare a conflict of interest.