Original ArticleDesigning idiosyncratic hmPCL-siRNA nanoformulated capsules for silencing and cancer therapy
Graphical abstract
Idiosyncratic mesoporous-PCL nanocapsules (hmPCL) have been synthesised through microemulsion technique and formulated with siRNA. Nanoformulated siRNA-hmPCL is a potential vector for siRNA mediated therapy and for knock down of infected cells.
Section snippets
Materials
hmPCL nanocapsules have been synthesised using commercially available PCL (Mw ~ 60,000, Tm ~ 60 °C, Sigma-Aldrich) through an ultrasonicated microemulsion approach using SiO2 nanoparticles and micelles of Igepal CO-520 (Mn ~ 441, density ~ 0.997 g/mL at 25 °C, b.p. ~ 240 °C, Sigma-Aldrich) as hard and soft templates, respectively.
Design of hollow-core-shell mesoporous PCL capsules (hmPCL)
Core-shell SiO2@PCL nanoparticles and hmPCL nanocapsules have been synthesised as discussed in the experimental section (supporting file). A schematic representation is shown in supporting Figure S1 for the formation of hmPCL nanocapsules. Figure 1, A and B showed the FESEM micrographs of spherical core-shell SiO2@PCL nanoparticles of size around ~ 200 nm (dia.) where PCL chains are deposited on SiO2 nanoparticles (~ 120-150 nm) (Figure S2, A and B at low and high magnification, respectively). The
Discussions
To achieve the hmPCL, the polymer chains are deposited layer-by-layer in a controlled manner using the ultrasonication onto the sacrificial SiO2 nanoparticles in the presence of a surfactant (Igepal CO-520) [(C2H4O)5 · (C15H24O)]. Removal of the template SiO2 nanoparticles and the surfactant resulted the formation of PCL nanocapsules with central hollow core of size 110-120 nm and mesoporous (avg. ~ 5-10 nm) shell with monodispersed in size. The release is controlled by the electrostatic interaction
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Cited by (0)
Any prior or upcoming presentation of abstracts at meetings regarding the research: None.
The authors acknowledge the financial support from the Department of Science and Technology (DST) for Fast-Track Grant for Young Scientist (Ref: SR/FTP/ETA-0079/2011) and School of Life Science, University of Hyderabad for cell culture facilities.