Original ArticleSilk fibroin nanoparticles support in vitro sustained antibiotic release and osteogenesis on titanium surface
Graphical Abstract
Immobilization of fibroin silk nanoparticles through silanization process on Ti surface increases the surface nano-roughness and hydrophilicity. Modified Ti surface exhibits increased osteoblast adhesion and growth. Antibiotic loaded nanoparticle immobilized surface shows sustained release of antibiotic and no deleterious effect on osteoblast adhesion. Such easy and cost-effective process of orthopedic implant surface modification can open new avenues for economically challenged patients.
Section snippets
Materials
Silk protein fibroin isolated from the glands of mature 5th instar larvae of Indian non-mulberry tasar silkworm (Antheraea mylitta) (IIT Kharagpur Farm, West Bengal, India), cellulose tubing of MWCO 12 kDa (Pierce, USA), acetone (Merck), alkaline phosphatase kit (Span diagnostics), APTES, o-phthaldialdehyde, gentamicin sulfate, glutaraldehyde, titanium foil (99.9% purity) Alizarin Red S (Sigma Aldrich, St. Louis, USA), Dulbecco’s modified Eagle’s medium (DMEM), trypsin, antibiotic solution and
Size and morphology of silk fibroin nanoparticles (SF-Np)
The mean radius of fabricated silk fibroin nanoparticles is found to be 90.63 ± 30 nm by dynamic light scattering analysis (Figure 1, A). Mean polydispersity index (PDI) of 0.2 is observed for the solution, which determines the uniformity of particle size in solution. TEM image of protein nanoparticles also supports the size and spherical morphology (Figure 1, B).
FTIR analysis of gentamicin loaded nanoparticles
FTIR is used to characterize the change in protein structure after loading with gentamicin (Figure 1, C). The characteristic absorption
Discussion
A bone/dental implant after surgery faces the altered microenvironment of damaged and diseased tissues. Moreover increased osseo-integration and decreased antibacterial activity seem to be a challenging task to achieve simultaneously. Failed osseo-integration requires additional surgeries, which further compromise the patient’s well being.12 To achieve successful implant integration, early osseo-integration and simultaneous antibacterial activity are assumed to be a preferred choice. Osteoblast
Author contributions
The manuscript was written through contributions of all authors. All authors have given approval to the final version of the manuscript. The authors declare no competing financial interest.
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Funding Sources: Department of Biotechnology (sanction no.: BT/PR10941/MED/32/333/2014) Govt. of India.